{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T20:29:00Z","timestamp":1785356940566,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,10]],"date-time":"2019-02-10T00:00:00Z","timestamp":1549756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["IR.TBZMED.REC."],"award-info":[{"award-number":["IR.TBZMED.REC."]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["1395.1360"],"award-info":[{"award-number":["1395.1360"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the association of <jats:italic>ERAP1<\/jats:italic> gene single nucleotide polymorphisms (SNPs) with the risk of ankylosing spondylitis (AS) and their role in modulation of the inflammatory interleukin (IL)\u201017\/IL\u201023 axis in the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For genotyping, 190 AS cases and 190 healthy controls were enrolled. After DNA extraction, all the subjects were genotyped for rs17482078, rs469876, and rs27038 polymorphisms using single specific primer polymerase chain reaction (PCR) assay. After isolation of peripheral blood mononuclear cells, RNA extraction and complementary DNA synthesis, real\u2010time PCR using SYBR Green master mix was employed to determine messenger RNA (mRNA) expression of IL\u201017A and IL\u201023 in PBMCs. Using enzyme\u2010linked immunosorbent assay, the concentration of these cytokines was determined in serum samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>It was observed that the A allele of rs27038 polymorphism significantly increased AS risk (odds ratio [OR] = 1.53, 95% CI =1.11\u20102.12; <jats:italic>P <\/jats:italic>= 0.0096). Moreover, AA and AG genotypes of this SNP were associated with increased (OR = 2.89, 95% CI = 1.42\u20105.85; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0031) and decreased (OR = 0.57, 95% CI = 0.36\u20100.92; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.021), respectively, risk of the disease. The rs27038 SNP was associated with C\u2010reactive protein level. There were significantly increased mRNA and serum concentrations of both IL\u201017A and IL\u201023 in AS patients compared with controls. Furthermore, AS patients with the AA in comparison to other genotypes for rs27038 SNP indicated significantly increased mRNA and serum concentration levels for both cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated the association of <jats:italic>ERAP1<\/jats:italic> gene rs27038 polymorphism with the risk of AS in an Iranian population. Additionally, it seems that rs27038 is involved in the modulation of the inflammatory IL\u201017\/IL\u201023 axis in AS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13494","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T05:46:08Z","timestamp":1549863968000},"page":"715-724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Susceptibility to <i>ERAP1<\/i> gene single nucleotide polymorphism modulates the inflammatory cytokine setting in ankylosing spondylitis"],"prefix":"10.1111","volume":"22","author":[{"given":"Maryam","family":"Hemmatzadeh","sequence":"first","affiliation":[{"name":"Connective Tissue Diseases Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Immunology, School of Medicine Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Student Research Committee Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Babaie","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Urmia University of Medical Sciences Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Ezzatifar","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Research Center, Faculty of Medicine Mazandaran University of Medical Sciences Sari Iran"},{"name":"Student Research Committee Mazandaran University of Medical Sciences Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh S.","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical School Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"Ebrazeh","sequence":"additional","affiliation":[{"name":"Department of Biology, Bonab Branch Islamic Azad University Bonab Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirin","family":"Golabi Aghdam","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrzad","family":"Hajaliloo","sequence":"additional","affiliation":[{"name":"Connective Tissue Diseases Research Center Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Azizi","sequence":"additional","affiliation":[{"name":"Non\u2010Communicable Diseases Research Center Alborz University of Medical Sciences Karaj Iran"},{"name":"Department of Immunology, School of Medicine Alborz University of Medical Sciences Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arezoo","family":"Gowhari Shabgah","sequence":"additional","affiliation":[{"name":"Immunology Research Center Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najibeh","family":"Shekari","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasrin","family":"Sehati","sequence":"additional","affiliation":[{"name":"Department of Genetic, School of Medicine Alborz University of Medical Sciences Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramin","family":"Hosseinzadeh","sequence":"additional","affiliation":[{"name":"Student Research Committee Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0736-6211","authenticated-orcid":false,"given":"Hamed","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Connective Tissue Diseases Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Immunology, School of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh","family":"Babaloo","sequence":"additional","affiliation":[{"name":"Connective Tissue Diseases Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Department of Immunology, School of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,10]]},"reference":[{"issue":"7","key":"e_1_2_7_2_1","first-page":"1384","article-title":"WHO\u2010ILAR COPCORD study (stage 1, urban study) in Iran","volume":"35","author":"Davatchi F","year":"2008","journal-title":"J Rheumatol"},{"issue":"6","key":"e_1_2_7_3_1","first-page":"S43","article-title":"Genetics of ankylosing spondylitis","volume":"20","author":"Brown M","year":"2002","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14397595.2016.1206174"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26500"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26250"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2015.1134511"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.097"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e32832b3795"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.05.092"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199803)41:3<460::AID-ART12>3.0.CO;2-X"},{"issue":"1","key":"e_1_2_7_12_1","first-page":"933","article-title":"Association of PTPN22 polymorphsims and ankylosing spondylitis susceptibility","volume":"8","author":"Meng Q","year":"2015","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.513"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23389"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp371"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kes254"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2007.17"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.01.014"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.85"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni859"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0213847"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.12.6814"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.12.050"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-010-1712-y"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-013-2824-y"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000013263"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/14397595.2016.1259716"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/key001"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2017.56"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.18502\/ijaai.v17i5.304"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.11.002"},{"issue":"4","key":"e_1_2_7_32_1","first-page":"294","article-title":"Association between endoplasmic reticulum aminopeptidase\u20101 (ERAP\u20101) and susceptibility to ankylosing spondylitis in Iran","volume":"11","author":"Mahmoudi M","year":"2012","journal-title":"Iran J Allergy Asthma Immunol"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290272"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.133"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-014-8576-2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.873"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003658"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000189"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24467"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-201783"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ket269"}],"container-title":["International Journal of Rheumatic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1756-185X.13494","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185X.13494","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1756-185X.13494","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185X.13494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T18:54:00Z","timestamp":1694285640000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1756-185X.13494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,10]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1111\/1756-185X.13494"],"URL":"https:\/\/doi.org\/10.1111\/1756-185x.13494","archive":["Portico"],"relation":{},"ISSN":["1756-1841","1756-185X"],"issn-type":[{"value":"1756-1841","type":"print"},{"value":"1756-185X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,10]]},"assertion":[{"value":"2018-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}