{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:46:10Z","timestamp":1789019170492,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the performance of a line blot (LB) assay for identifying anti\u2010PM\u2010Scl antibody (Ab).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We screened 56 serum samples from systemic sclerosis patients using a protein immunoprecipitation (IP) assay and an LB assay (Systemic Sclerosis Profile Euroline\u00ae Blot test kit) to detect anti\u2010PM\u2010Scl Ab. We compared the results obtained by the IP and LB assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 56 serum samples of SSc patients, 9 sera were positive for anti\u2010PM\u2010Scl75 Ab and 1 for anti\u2010PM\u2010Scl100 Ab by the LB assay. The protein IP assay revealed that none of the samples precipitated 75 or 100\u00a0kDa proteins identical to the anti\u2010PM\u2010Scl Ab reference serum, regardless of the positive or negative results obtained in the LB assay. Therefore, the false\u2010positive rates for both anti\u2010PM\u2010Scl75 Ab and anti\u2010PM\u2010Scl100 Ab by the LB assay were 100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Detection of anti\u2010PM\u2010Scl Ab assessed with a commercial LB assay requires extreme caution since it may yield false\u2010positive data.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13638","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T11:35:50Z","timestamp":1561635350000},"page":"1746-1751","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Performance evaluation of a line blot assay system for detection of anti\u2010PM\u2010Scl antibody in Japanese patients with systemic sclerosis"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5305-7770","authenticated-orcid":false,"given":"Yasuhito","family":"Hamaguchi","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Kuwana","sequence":"additional","affiliation":[{"name":"Department of Allergy and Rheumatology Nippon Medical School Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Takehara","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar628"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2014.07.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-015-0154-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2015.1077231"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-016-3494-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2016.12.014"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kex188"},{"key":"e_1_2_7_9_1","first-page":"751","article-title":"Progressive scleroderma and dermatomyositis","volume":"83","author":"Ricken D","year":"1977","journal-title":"Verh Dtsch Ges Inn Med"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar389"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.4049\/jimmunol.137.12.3802","article-title":"Immunolocalization and partial characterization of a nucleolar autoantigen (PM\u2010Scl) associated with polymyositis\/scleroderma overlap syndromes","volume":"137","author":"Reimer G","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370111"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08392.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0573-x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330508"},{"key":"e_1_2_7_16_1","first-page":"907","article-title":"Myositis-specific and myositis-associated autoantibodies in patient with dermatomyositis\/polymyositis; 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