{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:59:34Z","timestamp":1792799974151,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoarthritis (OA) is a prevalent chronic diseases characterized by formation of osteophytes and degradation of articular cartilage. Previous evidence has identified the regulatory effects of microRNAs (miRNAs) in OA. The goal of this study is to clearly explore the biological function of miR\u20101207\u20105p in OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MiR\u20101207\u20105p and C\u2010X3\u2010C motif chemokine receptor 1 (CX3CR1) expression in OA cartilages were revealed by accessing to Gene Expression Omnibus database. In vitro OA model was established by lipopolysaccharide (LPS) stimulation. Western blot and quantitative real\u2010time polymerase chain reaction were conducted to detect the expression level of genes. Cell counting kit\u20108 (CCK\u20108) and flow cytometric experiments were performed to investigate the proliferation and apoptosis capacities of CHON\u2010001 cells. Bioinformatics analysis was applied to predict the binding site of miR\u20101207\u20105p and CX3CR1, the connections of which were ascertained using luciferase reporter assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u20101207\u20105p expression was decreased while CX3CR1 was increased in OA cartilages. Up\u2010regulation of miR\u20101207\u20105p alleviated the LPS\u2010induced damage in the view of cell proliferation, apoptosis and extracellular matrix (ECM) degradation. A target of miR\u20101207\u20105p CX3CR1, its down\u2010regulation intensified the impacts of miR\u20101207\u20105p mimic, promoted proliferation and mitigated apoptosis. LPS exposure increased the protein expression of the phosphorylated I\u03baB\u03b1 and P65, and this phenomena was reversed due to miR\u20101207\u20105p up\u2010regulation and CX3CR1 knockdown. The treatment of Betulinic acid (BA; an activator of nuclear factor\u2010\u03baB pathway) reversed the miR\u20101207\u20105p mimic\u2010induced inhibitory effect on apoptosis in LPS\u2010treated CHON\u2010001.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results highlight that miR\u20101207\u20105p can prevent CHON\u2010001 from LPS\u2010stimulated injury, providing a novel biomarker for OA progression and further advancing treatment of OA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.13898","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T11:56:30Z","timestamp":1593431790000},"page":"1057-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["MiR\u20101207\u20105p\/CX3CR1 axis regulates the progression of osteoarthritis via the modulation of the activity of NF\u2010\u03baB 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