{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:42:26Z","timestamp":1787013746444,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Youth Medical Talent Project of Jiangsu","award":["QNRC2016214"],"award-info":[{"award-number":["QNRC2016214"]}]},{"name":"Key Technologies of Prevention and Control for Major and Infectious Diseases","award":["GWZX201604"],"award-info":[{"award-number":["GWZX201604"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Rheumatoid arthritis (RA) is an inflammatory disease, which seriously affects human joints. This study aimed to detect the changes in the expression of long non\u2010coding RNA growth arrest\u2010specific transcript 5 (GAS5) in peripheral blood mononuclear cells (PBMCs) derived from patients with RA and healthy controls (HC), as well as analyze the correlation between GAS5 and clinical indicators of RA. Also, the role and mechanism of GAS5 in regulating the AMP\u2010activated protein kinase (AMPK) pathway in RA was further assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The PBMCs were isolated from the RA patients. Next, GAS5 expression was detected in RA PBMCs by quantitative real\u2010time polymerase chain reaction, and its diagnostic value on RA was determined by receiver operating characteristic curves (ROC). The levels of interleukin (IL)\u20106 and IL\u201017 were detected via enzyme\u2010linked immunosorbent assay. The expressions of total and phosphorylated AMPK as well as p38MAPK were determined with Western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GAS5 was down\u2010regulated in RA PBMCs, and consequently serves as a potential diagnostic marker for RA (sensitivity, 90%; specificity, 80%; area under the curve, 0.89). Further, GAS5 negatively regulated erythrocyte sedimentation rate, C\u2010reactive protein, Disease Activity Score of 28 joints and antibodies against cyclic citrullinated peptide, as well as the IL\u20106 and IL\u201017 levels of RA PBMCs. Similarly, GAS5 was observed to activate the AMPK pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>GAS5 activated the AMPK pathway, while it negatively regulated the expression of cytokines IL\u20106 and IL\u201017.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.13930","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T09:54:23Z","timestamp":1596534863000},"page":"1318-1327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["LncRNA GAS5 activates the AMPK pathway in peripheral blood mononuclear cells derived from rheumatoid arthritis patients"],"prefix":"10.1111","volume":"23","author":[{"given":"Jijun","family":"Cao","sequence":"first","affiliation":[{"name":"The First People\u2019s Hospital of Taicang Jiangsu 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