{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:01:15Z","timestamp":1778108475499,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,7]],"date-time":"2021-02-07T00:00:00Z","timestamp":1612656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Genetic polymorphisms in the endoplasmic reticulum aminopeptidase gene\n                      <jats:italic>ERAP2<\/jats:italic>\n                      has been attributed with the etiopathogenesis of ankylosing spondylitis (AS). Here we assessed the association of\n                      <jats:italic>ERAP2<\/jats:italic>\n                      gene single nucleotide polymorphisms (SNPs) with AS predisposition in Iranian patients and determined their effect on the inflammatory state of the patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>For genotyping of rs2548538, rs2287988, and rs17408150 SNPs using a real\u2010time allelic discrimination approach, DNA was extracted from the whole blood of 250 AS patients and 250 healthy individuals. RNA of the peripheral blood mononuclear cells was separated, cDNA was synthesized, and transcriptional levels of cytokines, including interleukin (IL)\u201017A, IL\u201023, IL\u201010, and transforming growth factor\u2010\u03b2, were measured. Enzyme\u2010linked immunosorbent assay was used to measure the serum concentration on the cytokines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three\n                      <jats:italic>ERAP2<\/jats:italic>\n                      gene SNPs were not associated significantly with AS risk. Nonetheless, rs2287988 and rs17408150 SNPs showed statistically significant association with susceptibility to the disease in those AS patients who were positive for human leukocyte antigen (HLA)\u2010B27. Transcriptional level and serum concentration of IL\u201017A and IL\u201023 were higher, but those of IL\u201010 were lower in both AS patients and the HLA\u2010B27\u2010positive patient group relative to the control group. Nevertheless,\n                      <jats:italic>ERAP2<\/jats:italic>\n                      gene SNPs in the HLA\u2010B27\u2010positive AS patients did not affect the transcription level and serum concentration of cytokines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>ERAP2<\/jats:italic>\n                      gene rs2287988 and rs17408150 SNPs are associated with susceptibility to AS, but they are probably not determining the levels of IL\u201017A, IL\u201023, and IL\u201010 in this disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.14079","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T14:22:30Z","timestamp":1612794150000},"page":"567-581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Association of the genetic variants in the\n                    <i>endoplasmic reticulum aminopeptidase 2<\/i>\n                    gene with ankylosing spondylitis susceptibility"],"prefix":"10.1111","volume":"24","author":[{"given":"Mehrdad","family":"Ebrazeh","sequence":"first","affiliation":[{"name":"Department of Biology Bonab Branch Islamic Azad University Bonab Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Ezzatifar","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology Research Center Faculty of Medicine Mazandaran University of Medical Sciences Sari Iran"},{"name":"Student Research Committee Mazandaran 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Shabgah","sequence":"additional","affiliation":[{"name":"School of Medicine Bam University of Medical Sciences Bam Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Hemmatzadeh","sequence":"additional","affiliation":[{"name":"Student Research Committee Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed","family":"Aslani","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Medicine Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhad","family":"Babaie","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Urmia University of Medical Sciences Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhad","family":"Jadidi\u2010Niaragh","sequence":"additional","affiliation":[{"name":"Immunology Research Center Tabriz University of 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