{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:47:06Z","timestamp":1789987626161,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870615"],"award-info":[{"award-number":["81870615"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>MicroRNAs (miRNAs) have been shown to play a crucial role in inflammation regulation; however, their relationship with inflammation in acute gouty arthritis has not been fully elucidated. Herein, we conducted a study to explore the regulatory roles of miR\u2010223\u20103p and miR\u201022\u20103p in gouty\u2010associated inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In vitro and in vivo experiments were conducted to examine the molecular mechanisms of miRNA regulation in gouty inflammation. Dual\u2010luciferase reporter assay was used to verify the direct target of miR\u2010223\u20103p and miR\u201022\u20103p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that miR\u2010223\u20103p and miR\u201022\u20103p interacted with the 3\u2032 untranslated region segment of NLRP3 (nucleotide\u2010binding domain leucine\u2010rich repeat [NLR] and pyrin domain containing receptor 3) and inhibited its expression. A decreased expression of miR\u2010223\u20103p and miR\u201022\u20103p was observed in both mice air pouch synovium and phorbol myristrate acetate\u2010treated THP\u20101 cells stimulated with monosodium urate (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Compared with the negative control group, NLRP3 expression at the transcript and protein level in miR\u2010223\u20103p and miR\u201022\u20103p overexpression group significantly decreased after 6\u00a0hours of monosodium urate treatment in vivo and in vitro (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). The results of the dual\u2010luciferase reporter assay demonstrated that miR\u2010223\u20103p and miR\u201022\u20103p directly targeted NLRP3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings of the present study show that miR\u2010223\u20103p and miR\u201022\u20103p can reduce the inflammatory effects of gout by inhibiting the expression of NLRP3.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.14089","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T05:24:00Z","timestamp":1614662640000},"page":"599-607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["MiR\u2010223\u20103p and miR\u201022\u20103p inhibit monosodium urate\u2010induced gouty inflammation by targeting NLRP3"],"prefix":"10.1111","volume":"24","author":[{"given":"Xiang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology Affiliated Hospital of Medical College Qingdao University  Qingdao China"},{"name":"Transfusion Section Chongqing University Three Gorges Hospital  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwei","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Affiliated Hospital of Medical College Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingzi","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Affiliated Hospital of Medical College Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Affiliated Hospital of Medical College Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of 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