{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:31:28Z","timestamp":1792096288336,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970062"],"award-info":[{"award-number":["81970062"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770061"],"award-info":[{"award-number":["81770061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the novel key genes and biological processes that may lead to primary Sj\u00f6gren\u2019 s syndrome (pSS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We downloaded datasets about peripheral blood samples of pSS patients and healthy controls (GSE51092, GSE84844, and GSE66795) from Gene Expression Omnibus database. The weighted co\u2010expression network analysis and differential expression analysis first were implemented. After that, protein\u2013protein network interaction and Support Vector Machines were applied in the meantime to take intersection for key genes. Moreover, we conducted immune cell infiltration analysis to explore the relationship between the gene expression and concentration of immune cells in peripheral blood. Lastly, the expression of key genes was verified in pSS patients and murine models by reverse\u2010transcription polymerase chain reaction. Meanwhile, correlation analysis of gene expression and disease activity was also performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only 1 key gene, interferon induced with helicase c domain 1 (<jats:italic>IFIH1<\/jats:italic>), was identified to be both significantly up\u2010regulated and important for the diagnosis of pSS. The increased expression of <jats:italic>IFIH1<\/jats:italic> in peripheral blood was confirmed in data sets, patients and non\u2010obese diabetic (NOD) mice. Its expression was correlated with disease activity in patients as well. In addition, the <jats:italic>IFIH1<\/jats:italic> expression was also increased in spleen and salivary glands infiltrated with lymphocytes in NOD mice. Furthermore, immune cell infiltration analysis showed that the expression of <jats:italic>IFIH1<\/jats:italic> was positively correlated with the proportion of memory B cells and activated dendritic cells, and negatively correlated with the proportion of macrophage M0.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Here, bioinformatics analyses and experimental assays were performed to provide a new insight for understanding of pSS. <jats:italic>IFIH1<\/jats:italic> may be a new diagnostic marker or therapeutic target for pSS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.14668","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T02:10:26Z","timestamp":1679969426000},"page":"895-906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp><i>IFIH1<\/i><\/scp> was predicted as a key biomarker in primary Sj\u00f6gren's syndrome based on transcriptome analysis and experimental verification in patients and mice"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-2961","authenticated-orcid":false,"given":"Dan","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology Affiliated Drum Tower Hospital, Medical School, Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, Conversion Therapy Center for Hepatobiliary and Pancreatic Tumors, First Hospital of Jiaxing Affiliated Hospital of Jiaxing University  Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Affiliated Drum Tower Hospital, Medical School, Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Faculty of Medicine Macau University of Science and Technology  Macau SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4630-976X","authenticated-orcid":false,"given":"Genhong","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Affiliated Drum Tower Hospital, Medical School, Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Affiliated Drum Tower Hospital, Medical School, Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.04.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38726"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-209589"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40659"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874609808666150727112826"},{"issue":"3","key":"e_1_2_9_7_1","first-page":"185","article-title":"Type I interferon signature in Sj\u00f6gren's syndrome: pathophysiological and clinical implications","volume":"37","author":"Marketos N","year":"2019","journal-title":"Clin Exp Rheumatol"},{"issue":"4","key":"e_1_2_9_8_1","first-page":"3","article-title":"One year in review 2020: pathogenesis of primary Sj\u00f6gren's 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