{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T08:10:01Z","timestamp":1774512601013,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,9]],"date-time":"2023-07-09T00:00:00Z","timestamp":1688860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Previous studies have shown that human leukocyte antigen (HLA)\u2010B27 induces the accumulation of unfolded proteins in the endoplasmic reticulum (ER) to cause ER stress, resulting in the unfold protein response (UPR), apoptosis and autophagy. However, it is still unknown whether it affects the survival of monocytes. In this study, we attempted to examine the effect of HLA\u2010B27 gene knockout on the proliferation and apoptosis of THP\u20101 monocytic cell line and its potential mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HLA\u2010B27 gene knockout THP\u20101 cell line was constructed by lentivirus infection, and knockout efficiency was detected by immunofluorescence, quantitative reverse transcription \u2010 polymerase chain reaction (qRT\u2010PCR) and western blot. Cell Counting Kit\u20108 (CCK\u20108) method and Annexin\u2010V\/PI double staining were used to detect the proliferation and apoptosis of the constructed THP\u20101 cell line, respectively. qRT\u2010PCR was used to detect the effect of HLA\u2010B27 inhibition on the expressions of ER molecular chaperone binding immunoglobulin protein (BiP) and genes about the UPR pathway. The proliferation rate of human BiP protein\u2010stimulated THP\u20101 cells was detected by CCK\u20108 method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HLA\u2010B27 gene knockout THP\u20101 cells were successfully constructed by lentivirus infection. Knockout of HLA\u2010B27 effectively promoted the proliferation of THP\u20101 cells and inhibited the apoptosis induced by cisplatin. qRT\u2010PCR showed that BiP was synchronously increased, while activation of UPR pathway was inhibited. Stimulation with human BiP promoted the proliferation of THP\u20101 cells in a concentration\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HLA\u2010B27 inhibition can promote the proliferation and inhibit the apoptosis of THP\u20101 cells. The inhibition function may be achieved through promotion of BiP and inhibition of UPR pathway activation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.14758","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T05:38:36Z","timestamp":1688967516000},"page":"1474-1484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The study of the effect of <scp>HLA\u2010B27<\/scp> on <scp>THP<\/scp>\u20101 monocytic cells survival and its mechanism"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6750-142X","authenticated-orcid":false,"given":"Lian","family":"Gui","sequence":"first","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieruo","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of 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