{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T19:29:55Z","timestamp":1786562995248,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Rheumatoid arthritis (RA) is a kind of systemic autoimmune disease, and the joint inflammation and cartilage destruction are the major features. Some traditional Chinese medicine have been discovered to exhibit regulatory roles in the treatment of RA. Forsythiaside A (FA) as an active ingredient isolated from forsythia suspensa has been discovered to participate into the regulation of some diseases through improving inflammation. However, the regulatory effects of FA on the progression of RA keep indistinct.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>IL\u20101\u03b2 treatment (10\u2009ng\/mL) in MH7A cells was built to mimic RA in\u00a0vitro (cell) model. The cell viability was examined through CCK\u20108 assay. The cell proliferation was detected through Edu assay. The levels of TNF\u2010\u03b1, IL\u20106, and IL\u20108 were evaluated through ELISA. The protein expressions were measured through western blot. The cell apoptosis was assessed through flow cytometry. The cell migration and invasion abilities were tested through Transwell assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, it was revealed that the cell proliferation was strengthened after IL\u20101\u03b2 treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001), but this effect was reversed after FA treatment in a dose\u2010increasing manner (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). Furthermore, FA suppressed inflammation in IL\u20101\u03b2\u2010triggered MH7A cells through attenuating the levels of TNF\u2010\u03b1, IL\u20106, and IL\u20108 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). The cell apoptosis was lessened after IL\u20101\u03b2 treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001), but this effect was rescued after FA treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). Besides, the cell migration and invasion abilities were both increased after IL\u20101\u03b2 treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001), but these changes were offset after FA treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). Eventually, FA retarded the JAK\/STAT pathway through reducing p\u2010JAK\/JAK and p\u2010STAT\/STAT levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study manifested that FA exhibited anti\u2010migration and anti\u2010inflammation effects in RA in\u00a0vitro model (IL\u20101\u03b2\u2010triggered MH7A cells) through regulating the JAK\/STAT pathway. This work hinted that FA can be an effective drug for RA treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.14976","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T20:59:44Z","timestamp":1700859584000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Forsythiaside A exhibits anti\u2010migration and anti\u2010inflammation effects in rheumatoid arthritis in\u00a0vitro model"],"prefix":"10.1111","volume":"27","author":[{"given":"Minhui","family":"Su","sequence":"first","affiliation":[{"name":"Department of Rheumatology Changzhou Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine  Changzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinghua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Changzhou Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine  Changzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Changzhou Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine  Changzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Changzhou Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine  Changzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Changzhou Hospital of Traditional Chinese Medicine, Affiliated to Nanjing 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