{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T20:50:38Z","timestamp":1773348638643,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,25]],"date-time":"2024-02-25T00:00:00Z","timestamp":1708819200000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Psoriasis, a common chronic inflammatory skin condition, impacts around 2%\u20133% of the global population. Theacrine is recognized for its potential anti\u2010inflammatory and antioxidant properties. However, the role of theacrine in psoriasis remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purposes<\/jats:title><jats:p>To investigate the effects of theacrine on psoriasis and explore the underlying signaling pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For imiquimod (IMQ)\u2010induced Psoriasis\u2010like mice, the psoriatic inflammation was monitored using Psoriasis Area and Severity Index (PASI). The skin damage was observed using Hematoxylin and Eosin staining. The KI67 and CD4 in skin tissues were assessed using Immunohistochemistry analysis. Western blots were performed to evaluate the expression of Keratin 1 (KRT1), KRT6, LC3, P62, Beclin1, T\u2010bet, GATA3, RAR\u2010related orphan receptor (ROR)\u2010\u03b3t, Sirtuin\u20103 (SIRT3), Forkhead Box O3a (FOXO3a) and Parkin. Additionally, LC3B expression was analyzed using an immunofluorescent assay, while flow cytometry was performed to analyze the percentage of Th17, Th1, and Th2 positive cells in skin\u2010draining lymph node.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Theacrine improved skin condition by reducing hyperkeratosis and acanthosis, lowering PASI scores, and decreasing KI67\u2010positive cells. Theacrine also modulated keratin expression, elevating KRT1 while reducing KRT6 levels. Theacrine enhanced autophagy indicated by an increased LC3\u2010II\/LC3\u2010I ratio and Beclin1, while reduced P62 levels. Additionally, Theacrine reduced CD4\u2010positive cells and suppressed Th17 and Th1 cell activation. Theacrine activated the FOXO3a\/Parkin pathway by upregulating SIRT3 expression, and down\u2010regulation of SIRT3 counteracted theacrine's effects in psoriasis\u2010like mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Theacrine inhibits skin damage, promotes autophagy, and mediates inflammation in IMQ\u2010induced psoriasis mice via upregulating SIRT3 to activate FOXO3a\/Parkin pathway, positioning theacrine as a candidate for psoriasis treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.15085","type":"journal-article","created":{"date-parts":[[2024,2,25]],"date-time":"2024-02-25T08:25:42Z","timestamp":1708849542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Theacrine enhances autophagy and inhibits inflammation via regulating <scp>SIRT3<\/scp>\/<scp>FOXO3a<\/scp>\/Parkin pathway"],"prefix":"10.1111","volume":"27","author":[{"given":"Jin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenliang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongshan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2067-8304","authenticated-orcid":false,"given":"Fang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Jinling 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effects of a theacrine\u2010based supplement on mRNAs related to various metabolic processes and sirtuin activity in\u00a0vitro.2020.","DOI":"10.21203\/rs.3.rs-51095\/v1"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.12.060"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.15586\/aei.v50i6.683"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2020.152006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI97426"}],"container-title":["International Journal of Rheumatic 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