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We conducted molecular docking to validate the interaction between the key active ingredients of HQC and the selected core targets associated with the PI3K\/AKT signaling pathway, and employed data mining to delve deeper into the correlation between HQC usage and the improvement of clinical immunoinflammatory indicators. Additionally, AS\u2010PBMCs and FLS co\u2010culture cell models will be employed to further explore the mechanism of action of HQC in improving AS immune inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Based on network pharmacology core target prediction, TNF\u2010\u03b1, IL\u201017A, IL\u20106, IL\u201010, and AKT1 were identified as core targets, and KEGG enrichment analysis suggested that PI3K\/AKT, IL\u201017, and TNF might be key signaling pathways of HQC in improving immune inflammation in AS. The results of molecular docking demonstrated a favorable binding energy between the selected AKT1 core target and the key active components of HQC, including baicalin, baicalein,\u00a0and quercetin. Clinical data mining revealed a significant correlation between HQC use and improvement in clinical immunoinflammatory indexes, such as ESR, CRP, IgA, IgM, IgG, C3, C4, patient perception scores (VAS, SAS, SDS), and (PF, SF) scores. In\u00a0vitro cellular experiments further supported the significant ability of HQC to inhibit PI3K\/AKT signaling pathway activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HQC, as an herbal compound preparation, exhibits anti\u2010inflammatory effects through multiple components and targets, making it a potential treatment option for AS. Furthermore, an underlying mechanism of action of HQC is its ability to ameliorate immune inflammation in AS patients by inhibiting the PI3K\/AKT signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.15229","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T04:29:43Z","timestamp":1719030583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanistic study on the improvement of immune inflammation in ankylosing spondylitis through regulation of the PI3K\/AKT signaling pathway by Huangqin Qingre Chubi capsules"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8842-1845","authenticated-orcid":false,"given":"Chengzhi","family":"Cong","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Anhui University of Chinese Medicine  HeFei China"},{"name":"First Clinical Medical College Anhui 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