{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T22:12:30Z","timestamp":1788473550993,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2019MS08180"],"award-info":[{"award-number":["2019MS08180"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160424"],"award-info":[{"award-number":["82160424"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoarthritis (OA) is a prevalent degenerative disease. We explored the role and regulatory mechanisms of lncRNA\u2010FAS\u2010AS1 in OA progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We exposed human immortalized chondrocytes to IL\u20101\u03b2 for 24\u2009h to induce an OA cell model. The target molecule levels were assessed using western blot and quantitative real\u2010time PCR (RT\u2010qPCR). Cell viability and apoptosis were measured using CCK\u20108 and flow cytometry. The m6A modification of FAS\u2010AS1 was determined using MeRIP. We examined the binding relationships between FAS\u2010AS1, Fragile X mental retardation 1 (FMR1), and A disintegrin and metalloproteinase 8 (ADAM8) using RIP and RNA pull\u2010down. The OA animal model was established by separating the medial collateral ligament and medial meniscus. Safranin\u2010O staining and Mankin's scale were employed to evaluate pathological changes within the cartilage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FAS\u2010AS1, METTL14, and ADAM8 were upregulated, and the JAK\/STAT3 signaling pathway was activated in OA mice and IL\u20101\u03b2\u2010induced chondrocytes. FAS\u2010AS1 knockdown inhibited extracellular matrix degradation in IL\u20101\u03b2\u2010induced chondrocytes; however, ADAM8 overexpression reversed this effect. FAS\u2010AS1 maintained the stability of ADAM8 mRNA by recruiting FMR1. METTL14 knockdown repressed FAS\u2010AS1 expression in an m6A\u2010dependent manner. FAS\u2010AS1 overexpression reversed the inhibitory effects of METTL14 knockdown on JAK\/STAT3 signaling and cartilage damage in the OA model both in\u00a0vitro and in\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>METTL14\u2010mediated FAS\u2010AS1 promotes OA progression through the FMR1\/ADAM8\/JAK\/STAT3 axis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.15323","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T12:29:46Z","timestamp":1725280186000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["METTL14\u2010mediated lncRNA\u2010FAS\u2010AS1 promotes osteoarthritis progression by up\u2010regulating ADAM8"],"prefix":"10.1111","volume":"27","author":[{"given":"Zhehua","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Third Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia BaoGang Hospital  Baotou Inner Mongolia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honggang","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Third Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia BaoGang Hospital  Baotou Inner Mongolia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Experimental Center The Third Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia BaoGang Hospital  Baotou Inner Mongolia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Third Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia BaoGang Hospital  Baotou Inner Mongolia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6047-7299","authenticated-orcid":false,"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Third Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia BaoGang Hospital  Baotou Inner Mongolia China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052767"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(19)30417-9"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01330-w"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073594"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-021-00710-y"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15907"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43440-019-00015-y"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1130823"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/cad.0000000000000790"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-019-0443-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2020-0184"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10011"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2673-7"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2021.1919838"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202009045"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abm6265"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-023-03505-1"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-35502-z"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/09674845.2020.1765459"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2023.148035"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201805_15051"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.14336\/ad.2019.0814"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2020.06.006"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010817"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/13854046.2016.1184652"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2022.2108633"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-020-00295-8"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.946219"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/19476035231200336"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01228-8"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112589"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-021-00696-7"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00613"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.25.15521"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddy292"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161209788682361"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2741"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1110765"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1069057"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.111034"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/os.12690"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2021.210402"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110349"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2022.02.002"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345231180464"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05391-7"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14220"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110740"}],"container-title":["International Journal of Rheumatic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185X.15323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T13:55:37Z","timestamp":1727358937000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1756-185X.15323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/1756-185X.15323"],"URL":"https:\/\/doi.org\/10.1111\/1756-185x.15323","archive":["Portico"],"relation":{},"ISSN":["1756-1841","1756-185X"],"issn-type":[{"value":"1756-1841","type":"print"},{"value":"1756-185X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2024-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15323"}}