{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:32:20Z","timestamp":1792625540854,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The main objective of this study is to elucidate the role of CXCR1 Ab, IL\u201017 Ab, and gentamicin in protecting septic arthritis by regulating neutrophil functional responses while evaluating the contribution of the CXCL8\u2010CXCR1 pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eighty\u2010four experimental swiss albino mice were utilized to study septic arthritis. They were divided into eight groups. After developing sepsis, respective mice groups were treated with CXCR1 Ab, IL\u201017 Ab, and gentamicin doses. Doses were administered on days 1, 8, and 13 of the experimental schedule. At the early, middle, and late phases of the experiment i.e. at 3, 10, and 15 DPI (Days Post Infection), mice were sacrificed and blood and tissues were collected for further experimental evaluations. Different functional studies were performed on isolated blood neutrophils, spleen, and synovial tissues. Histological evaluation, immunofluorescence study, sepsis profile, and downstream signaling pathway analysis were done to obtain data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Infected mice group exhibited high inflammatory responses while treatment helped to mitigate them. IL\u201017 neutralization helped to lower bacterial burden, neutrophil ROS activity, MPO activity, and ALP activity. However, the combined neutralization of CXCR1 and IL\u201017 greatly influenced PMN chemotactic activity and lysozyme activity. At the early phase of the experiment, IL\u201017 neutralization's impact was more prominent, while later, CXCR1 neutralization gained the upper hand.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We can conclude that IL\u201017 Ab in combination with gentamicin is potent in modulating neutrophil activities positively to cure sepsis, while CXCR1 Ab, through the CXCL8\/CXCR1 pathway, regulates neutrophil functional activities by impacting different downstream signaling cascades.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.70144","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:06:01Z","timestamp":1744088761000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Neutralization of\n                    <scp>IL<\/scp>\n                    \u201017 and\n                    <scp>CXCR1<\/scp>\n                    Protects Septic Arthritis by Regulating\n                    <scp>CXCL8<\/scp>\n                    \u2010\n                    <scp>CXCR1<\/scp>\n                    Pathway Along With Functional Activities in Neutrophils"],"prefix":"10.1111","volume":"28","author":[{"given":"Sharmistha","family":"Ghosh","sequence":"first","affiliation":[{"name":"Immunology Laboratory, Department of Physiology University of Calcutta, University Colleges of Science and Technology  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8603-8621","authenticated-orcid":false,"given":"Biswadev","family":"Bishayi","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Department of Physiology University of Calcutta, University Colleges of Science and Technology  Kolkata West Bengal 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