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The critical role of neutrophils in these conditions is increasingly recognized. This study aimed to identify a shared set of neutrophils differentially expressed genes (NDEGs) to aid in the differential diagnosis of the two diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatics analysis of GEO data combined with WGCNA identified 65 key NDEGs. Functional enrichment and immune infiltration analyses were conducted. RT\u2010qPCR validated six hub NDEGs in neutrophils from IBD and BD patients. Serum CD226 levels were measured by ELISA, and a ROC curve assessed its diagnostic value. Additionally, neutrophils were stimulated with patient serum, followed by Western blot analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Immune infiltration analysis showed higher blood neutrophil levels in BD than in IBD. Neutrophil sequencing identified NDEGs upregulated in BD but downregulated in IBD, linked to T\u2010cell receptor pathways. RT\u2010PCR confirmed elevated FYN, CD99, SKAP1, and CD226 in BD neutrophils, while KLRG1 and MATK were higher in IBD. ELISA showed increased serum CD226 in BD. Western blot revealed higher Elastase and PAD4 in BD\u2010stimulated neutrophils, while CXCL11 was elevated in IBD\u2010stimulated neutrophils.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggest that BD and IBD neutrophils may have distinct functional states, potentially linked to differential T\u2010cell interactions. These insights highlight neutrophils' diverse roles in immune dysregulation and their potential as diagnostic markers and therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.70229","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T09:06:07Z","timestamp":1745226367000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated Analysis of Intersecting Neutrophil Signatures in Beh\u00e7et's Disease and Inflammatory Bowel Disease"],"prefix":"10.1111","volume":"28","author":[{"given":"Pengchong","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Digestive Health, Department of Gastroenterology Beijing Digestive Disease Center, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease  Beijing 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