{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:24:08Z","timestamp":1782577448768,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Non\u2010radiographic axial spondyloarthritis (nr\u2010axSpA) is an early stage of axial spondyloarthritis characterized by the absence of definitive radiographic changes. Interleukin\u201017 inhibitors (IL\u201017i) have shown efficacy in treating nr\u2010axSpA, but a significant proportion of patients fail to respond. Identifying predictive biomarkers for IL\u201017i response is crucial for optimizing treatment strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This retrospective study analyzed pre\u2010treatment serum samples from nr\u2010axSpA patients treated with secukinumab. Responders (R) and non\u2010responders (NR) were defined based on changes in ASDAS\u2010CRP scores after 12\u2009weeks of therapy. Serum protein profiles were analyzed using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) with label\u2010free quantification, followed by validation of candidate proteins using enzyme\u2010linked immunosorbent assay (ELISA). Statistical analyses included Student's\n                      <jats:italic>t<\/jats:italic>\n                      \u2010test, Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      \u2010test, and Spearman's correlation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LC\u2013MS\/MS identified 47 differentially expressed proteins (DEPs) between R and NR groups, with 31 upregulated and 16 downregulated in NR. Seven proteins including heat shock protein HSP 90\u2010alpha (HSP90\u03b1) and serotransferrin were selected for ELISA validation. ELISA confirmed significantly higher HSP90\u03b1 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.027) and lower serotransferrin (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.034) levels in NR compared to R. HSP90\u03b1 showed a mild negative correlation with the reduction of BASDAI scores (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.30,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.035), while serotransferrin exhibited a mild positive correlation (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.287,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.048). Both proteins correlate mildly with the disease activity index.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Serum levels of HSP90\u03b1 and serotransferrin may serve as potential biomarkers for predicting IL\u201017i treatment response in nr\u2010axSpA patients. Elevated HSP90\u03b1 and reduced serotransferrin levels may reflect chronic inflammation and disease activity, particularly from a subjective symptom perspective. Further validation in prospective studies is needed to confirm their utility in guiding personalized treatment strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.70303","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T01:46:02Z","timestamp":1750815962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>HSP<\/scp>\n                    90\u2010Alpha and Serotransferrin as Potential Biomarkers for Predicting\n                    <scp>IL<\/scp>\n                    \u201017 Inhibitor Response in Non\u2010Radiographic Axial Spondyloarthritis: A Retrospective Analysis"],"prefix":"10.1111","volume":"28","author":[{"given":"Linkai","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3293-0689","authenticated-orcid":false,"given":"Xubin","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiduo","family":"Xu","sequence":"additional","affiliation":[{"name":"Shenzhen Longhua Institute of Immunology Transformation  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9953-1315","authenticated-orcid":false,"given":"Xianghui","family":"Wen","sequence":"additional","affiliation":[{"name":"Shenzhen Longhua Institute of Immunology Transformation  Shenzhen China"},{"name":"Department of Rheumatology Shenzhen Longhua District Central Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1818-3605","authenticated-orcid":false,"given":"Xuqi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liudan","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zetao","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shude","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieruo","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"},{"name":"Shenzhen Longhua Institute of Immunology Transformation  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.108217"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.108233"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2022.105468"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.24025"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard\u20102022\u2010223296"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11051328"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1759720X241284869"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.988532"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BIO20200057"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR118.001131"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.113.031591"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa498"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010022\u201000175\u2010x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13020251"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12070911"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2013.07.013"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6248"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21176401"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094591"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u2010981\u201013\u20109589\u20105_2"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.2012"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2021.02.010"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2021.05.001"}],"container-title":["International Journal of Rheumatic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185X.70303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1756-185X.70303","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185X.70303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:12:18Z","timestamp":1782123138000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1756-185X.70303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/1756-185X.70303"],"URL":"https:\/\/doi.org\/10.1111\/1756-185x.70303","archive":["Portico"],"relation":{},"ISSN":["1756-1841","1756-185X"],"issn-type":[{"value":"1756-1841","type":"print"},{"value":"1756-185X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"2025-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70303"}}