{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T10:13:59Z","timestamp":1777544039195,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim is to pinpoint crucial genes linked to CD8<jats:sup>+<\/jats:sup> T cells in rheumatoid arthritis (RA) for aiding in diagnosis, predicting disease progression, and ultimately discovering potential drug targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study utilized datasets from the Gene Expression Omnibus (GEO) database to analyze gene expression profiles in RA patients. Weighted Gene Coexpression Network Analysis (WGCNA) was conducted to identify gene modules associated with the diseases, followed by differential gene expression analysis. Functional enrichment and protein\u2013protein interaction (PPI) network analysis were employed. Gene Set Enrichment Analysis (GSEA) and Disease Ontology (DO) analysis were applied to understand their potential pathways. Transcription factors (TFs) correlated with target gene expression were screened, and TF binding sites were predicted. The proportion of immune cell infiltration was calculated using CIBERSORT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study identified 58 candidate genes associated with CD8<jats:sup>+<\/jats:sup> T cells in RA. The top five genes, <jats:italic>RSAD2, IFIT3, OAS1, IFIT2<\/jats:italic>, and <jats:italic>SAMD9L<\/jats:italic>, were found to be upregulated in RA and other autoimmune diseases. <jats:italic>IFIT3<\/jats:italic> showed potential diagnostic value in RA, with significant expression differences in RA vs. OA samples. TF <jats:italic>BCL11B<\/jats:italic> bound to the <jats:italic>IFIT3<\/jats:italic> promoter. GSEA analysis indicated <jats:italic>IFIT3<\/jats:italic>'s influence on pathways like the cell cycle and TNF signaling. Immune landscape analysis showed <jats:italic>IFIT3<\/jats:italic>'s correlation with immune cell infiltration such as B cells and plasma cells. Drug prediction analysis suggested naringin's treatment potential for RA via targeting <jats:italic>IFIT3<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study identifies key CD8<jats:sup>+<\/jats:sup> T\u2010cell genes in RA, with <jats:italic>IFIT3<\/jats:italic> as a potential diagnostic and therapeutic target, revealing <jats:italic>BCL11B<\/jats:italic>'s regulatory role.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1756-185x.70385","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T11:46:46Z","timestamp":1755085606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Potential <scp>CD8<\/scp><sup>+<\/sup> T\u2010Cell\u2010Related Biomarker <scp><i>IFIT3<\/i><\/scp> for Rheumatoid Arthritis"],"prefix":"10.1111","volume":"28","author":[{"given":"Kangsong","family":"Tian","sequence":"first","affiliation":[{"name":"West Hospital Orthopaedic Trauma Department Zibo Central Hospital  Zibo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Guo","sequence":"additional","affiliation":[{"name":"Health Care Department Zibo Infectious Disease Hospital  Zibo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Yan","sequence":"additional","affiliation":[{"name":"West Hospital Orthopaedic Trauma Department Zibo Central Hospital  Zibo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6432-895X","authenticated-orcid":false,"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Orthopedics Department Zibo Infectious Disease Hospital  Zibo China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10112857"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.689698"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2669"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2020.102735"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u201001048\u20104"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00856"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.74"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109902"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.799440"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20109\u2010559"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu684"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.045"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3337"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010023\u201004516\u20106"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012\u2010024\u201009937\u20103"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1591164"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2019.1676825"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361\u20106560\/aab4b1"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34453"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.70"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.5613"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14091919"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2023.103453"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.30332"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.111501"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40576"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.5556"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075\u2010020\u20102137\u2010y"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28259"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2475"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1610617114"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.88.72"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22918"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.08.032"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183928"}],"container-title":["International Journal of Rheumatic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1756-185x.70385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T07:40:59Z","timestamp":1756366859000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1756-185x.70385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/1756-185x.70385"],"URL":"https:\/\/doi.org\/10.1111\/1756-185x.70385","archive":["Portico"],"relation":{},"ISSN":["1756-1841","1756-185X"],"issn-type":[{"value":"1756-1841","type":"print"},{"value":"1756-185X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2025-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70385"}}