{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T12:00:44Z","timestamp":1784980844194,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study sought to investigate the mechanism by which tofacitinib affects inflammatory response and osteogenic differentiation in immune\u2010mediated inflammatory arthritis in rats in relation to Janus kinase (JAK)\/signal transducer and activator of transcription 3 (STAT3).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Wistar male rats were induced by the combination of bovine proteoglycan and incomplete\/complete Freund's adjuvant to establish the immune\u2010mediated inflammatory arthritis model. The clinical manifestations were observed. Hematoxylin and eosin staining was used to evaluate pathological changes in sacroiliac joints and surrounding cartilage. Additionally, the levels of osteogenic differentiation\u2010related proteins, serum inflammatory factors, and alkaline phosphatase (ALP) were measured. Flow cytometry was conducted to analyze the proportion of Th17 cells in peripheral blood. Alizarin red staining detected the osteogenic differentiation of ligament fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The rats exhibited joint swelling, inflammatory infiltration in the sacroiliac joint, and cartilage destruction after modeling. Elevations were also detected in the levels of serum pro\u2010inflammatory factors, the proportion of Th17 cells, ALP activity, phosphorylated (p)\u2010JAK1\/2, p\u2010STAT3, osteogenic differentiation markers in ligament fibroblasts, as well as in the mineralized nodules. Tofacitinib intervention inhibited the phosphorylation of JAK and STAT3, downregulated bone differentiation\u2010related proteins, decreased the mineralized nodules, reduced the activity of ALP, and decreased the levels of pro\u2010inflammatory factors. However, the combined use of the STAT3 agonist Colivelin partially reversed the inhibitory effect of tofacitinib on joint inflammation and osteogenic differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Tofacitinib alleviated the inflammatory response in immune\u2010mediated inflammatory arthritis and inhibited osteogenic differentiation in rats via inhibition of the JAK\/STAT3 signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.70773","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:14:02Z","timestamp":1784099642000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>JAK<\/scp>\n                    Inhibitor Tofacitinib Ameliorates Inflammation Response and Osteogenic Differentiation in Immune\u2010Mediated Inflammatory Arthritis in Rats"],"prefix":"10.1111","volume":"29","author":[{"given":"Zhiming","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genlian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Preventive Treatment of Disease The Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Sanming Integrated Traditional Chinese and Western Medicine Hospital  Sanming Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congrong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiafang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongbin","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongshan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The Second Affiliated Hospital of Fujian Traditional Chinese Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2902-8240","authenticated-orcid":false,"given":"Feng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Fuzhou Second General Hospital  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2014.01.015"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.822582"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.133"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.935534"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.14956.1"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.21037\/apm-20-277"},{"issue":"2","key":"e_1_2_12_8_1","first-page":"318","article-title":"The Mechanism of Action of Tofacitinib\u2014An Oral Janus Kinase Inhibitor for the Treatment of Rheumatoid Arthritis","volume":"34","author":"Hodge J. 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