{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:00:24Z","timestamp":1790150424195,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Rheum Dis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the therapeutic efficacy of human umbilical cord\u2010derived mesenchymal stem cells (hUC\u2010MSCs) in a murine model of autoimmune premature ovarian insufficiency (POI), and to investigate the association between ubiquitin\u2010conjugating enzyme E2N (UBE2N) expression and disease progression as well as treatment response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Autoimmune POI was induced in female BALB\/c mice by zona pellucida glycoprotein 3 peptide (pZP3) immunization. Thirty mice were randomly assigned to Control, POI Model, and hUC\u2010MSC Treatment groups. Treated mice received weekly tail vein injections of hUC\u2010MSCs (1\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      cells\/mouse) for 4\u2009weeks. Estrous cyclicity, fertility, ovarian histopathology, peripheral Th17\/Treg balance, and the expression of IL\u201017, IFN\u2010\u03b3, and UBE2N were evaluated by immunohistochemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with the POI Model group, hUC\u2010MSC treatment restored regular estrous cyclicity, improved ovarian morphology, and increased litter size. Flow cytometry showed correction of the abnormal Th17\/Treg imbalance in treated mice. Immunohistochemistry revealed marked upregulation of UBE2N, IL\u201017, and IFN\u2010\u03b3 in ovarian tissue from POI mice, whereas hUC\u2010MSC administration significantly reduced their expression. No treatment\u2010related adverse effects were observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>hUC\u2010MSCs effectively alleviated ovarian dysfunction in autoimmune POI mice. The therapeutic improvement was accompanied by systemic immune rebalancing and decreased aberrant ovarian UBE2N expression, indicating UBE2N as a candidate inflammatory biomarker associated with autoimmune ovarian injury and immune dysregulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1756-185x.70822","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:00:42Z","timestamp":1790082042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Umbilical Cord Mesenchymal Stem Cells Alleviate Autoimmune\n                    <scp>POI<\/scp>\n                    Associated With Downregulated\n                    <scp>UBE2N<\/scp>\n                    \u2010Mediated Ovarian Inflammation"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0592-1043","authenticated-orcid":false,"given":"Jinwei","family":"Li","sequence":"first","affiliation":[{"name":"Shenzhen Huishan Biotechnology co., Ltd.  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