{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:42:26Z","timestamp":1787013746420,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T00:00:00Z","timestamp":1385337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["477231\/2012-8"],"award-info":[{"award-number":["477231\/2012-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Summary<\/jats:title>\n \n Brazilian sugarcane has been shown to obtain part of its nitrogen via biological nitrogen fixation (\n BNF<\/jats:styled-content>\n ). Recent reports, based on the culture independent sequencing of bacterial\n \n nifH<\/jats:italic>\n <\/jats:styled-content>\n complementary\n DNA<\/jats:styled-content>\n (\n cDNA<\/jats:styled-content>\n ) from sugarcane tissues, have suggested that members of the\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n genus could play a role in sugarcane\u2010associated\n BNF<\/jats:styled-content>\n . Here we report on the isolation of\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n spp. isolates and a few other species from roots of sugarcane cultivar\n RB<\/jats:styled-content>\n 867515 by two cultivation strategies: direct isolation on culture media and capture of\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n spp. using the promiscuous legume\n \n V<\/jats:italic>\n <\/jats:styled-content>\n igna unguiculata<\/jats:italic>\n as trap\u2010plant. Both strategies permitted the isolation of genetically diverse\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n spp. isolates, as concluded from enterobacterial repetitive intergenic consensus polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) fingerprinting and 16S ribosomal\n RNA<\/jats:styled-content>\n ,\n \n nifH<\/jats:italic>\n <\/jats:styled-content>\n and\n \n nodC<\/jats:italic>\n <\/jats:styled-content>\n sequence analyses. Several isolates presented\n \n nifH<\/jats:italic>\n <\/jats:styled-content>\n phylotypes highly similar to\n \n nifH<\/jats:italic>\n cDNA\n <\/jats:styled-content>\n phylotypes detected in field\u2010grown sugarcane by a culture\u2010independent approach. Four isolates obtained by direct plate cultivation were unable to nodulate\n \n V<\/jats:italic>\n <\/jats:styled-content>\n .\u2009unguiculata<\/jats:italic>\n and, based on\n PCR<\/jats:styled-content>\n analysis, lacked a\n \n nodC<\/jats:italic>\n <\/jats:styled-content>\n gene homologue. Acetylene reduction assay showed\n in vitro<\/jats:italic>\n nitrogenase activity for some\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n spp. isolates, suggesting that these bacteria do not require a nodule environment for\n BNF<\/jats:styled-content>\n . Therefore, this study brings further evidence that\n \n B<\/jats:italic>\n <\/jats:styled-content>\n radyrhizobium<\/jats:italic>\n spp. may play a role in sugarcane\u2010associated\n BNF<\/jats:styled-content>\n under field conditions.\n <\/jats:p>","DOI":"10.1111\/1758-2229.12122","type":"journal-article","created":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T07:53:50Z","timestamp":1383292430000},"page":"354-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Endophytic\n \n B<\/i>\n <\/scp>\n radyrhizobium<\/i>\n spp. isolates from sugarcane obtained through different culture strategies"],"prefix":"10.1111","volume":"6","author":[{"given":"Luc Felicianus Marie","family":"Rouws","sequence":"first","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakson","family":"Leite","sequence":"additional","affiliation":[{"name":"Instituto de Agronomia Departamento de Solos Universidade Federal Rural do Rio de Janeiro BR 465 km 7 23890\u2010000 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Feitosa","family":"de Matos","sequence":"additional","affiliation":[{"name":"Instituto de Agronomia Departamento de Solos Universidade Federal Rural do Rio de Janeiro BR 465 km 7 23890\u2010000 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerri Edson","family":"Zilli","sequence":"additional","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia Reed Rodrigues","family":"Coelho","sequence":"additional","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Ribeiro","family":"Xavier","sequence":"additional","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doreen","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department Environmental Genomics Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health Ingolstaedter Landstrasse 1 85764 Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department Microbe\u2010Plant Interactions Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health Ingolstaedter Landstrasse 1 85764 Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica Massena","family":"Reis","sequence":"additional","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ivo","family":"Baldani","sequence":"additional","affiliation":[{"name":"Embrapa Agrobiologia BR 465 km 7 23891\u2010460 Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0765.2005.tb00034.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2012.07.008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0001-37652005000300014"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2012.08.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00012\u201011"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00238.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02370096"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5437-5447.2000"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02384.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01400455"},{"key":"e_1_2_5_12_1","first-page":"310","article-title":"Recent changes in concepts of plant bacteria interactions: endophytic N2 fixing bacteria","volume":"44","author":"D\u00f6bereiner J.","year":"1992","journal-title":"Ci\u00eancia e Cultura"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-011-0812-0"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139548"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4290(99)00087-8"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1982.tb04841.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/256407a0"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-29298-5_107"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-4-981"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.16.5384-5391.2004"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(87)90077-0"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1890\/06-0219"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.028803-0"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02090.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcs150"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-006-0025-0"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/256406a0"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3103-3110.2006"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.053082\u20100"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02879-0"},{"key":"e_1_2_5_32_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou M.H.","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2005.02.015"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-204X2012000200015"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fcr.2005.01.017"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-010-0557-1"},{"key":"e_1_2_5_37_1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1128\/aem.60.2.581-586.1994","article-title":"A selective medium for the isolation and quantification of Bradyrhizobium japonicum and Bradyrhizobium elkanii strains from soils and inoculants","volume":"60","author":"Tong Z.","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj1992.03615995005600010017x"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-011-1016-3"},{"key":"e_1_2_5_40_1","volume-title":"A manual for the practical study of root\u2010nodule bacteria. IBP Handbook no. 15","author":"Vincent J.M.","year":"1970"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2012.10.009"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.7.1927-1931.1993"}],"container-title":["Environmental Microbiology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1758-2229.12122","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1758-2229.12122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T21:59:56Z","timestamp":1761861596000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1758-2229.12122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,25]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/1758-2229.12122"],"URL":"https:\/\/doi.org\/10.1111\/1758-2229.12122","archive":["Portico"],"relation":{},"ISSN":["1758-2229","1758-2229"],"issn-type":[{"value":"1758-2229","type":"print"},{"value":"1758-2229","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,25]]},"assertion":[{"value":"2013-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}