{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:20:44Z","timestamp":1790817644388,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T00:00:00Z","timestamp":1437609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council (NERC)","doi-asserted-by":"crossref","award":["NE\/F019254\/1"],"award-info":[{"award-number":["NE\/F019254\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/M011305\/1"],"award-info":[{"award-number":["BB\/M011305\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004117","name":"A.G. Leventis Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004117","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2015,12]]},"abstract":"Summary<\/jats:title>\n \n Species belonging to the filamentous cyanobacterial genus\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n are responsible for a significant fraction of oceanic nitrogen fixation. The availability of phosphorus (\n P<\/jats:styled-content>\n ) likely constrains the growth of\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n in certain regions of the ocean. Moreover,\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n species have recently been shown to play a role in an emerging oceanic phosphorus redox cycle, further highlighting the key role these microbes play in many biogeochemical processes in the contemporary ocean. Here, we show that\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium erythraeum<\/jats:italic>\n \u2005\n IMS101<\/jats:styled-content>\n can grow on the reduced inorganic compound phosphite as its sole source of\n P<\/jats:styled-content>\n . The components responsible for phosphite utilization are identified through heterologous expression of the\n \n T<\/jats:italic>\n <\/jats:styled-content>\n . erythraeum<\/jats:italic>\n \u2005\n \n IMS101\n T<\/jats:italic>\n <\/jats:styled-content>\n ery_0365\u20130368<\/jats:italic>\n genes, encoding a putative adenosine triphosphate (ATP)\u2010binding cassette transporter and nicotinamide adenine dinucleotide (NAD)\u2010dependent dehydrogenase, in the model cyanobacteria\n \n S<\/jats:italic>\n <\/jats:styled-content>\n ynechocystis<\/jats:italic>\n sp.\n PCC6803<\/jats:styled-content>\n . We demonstrate that only combined expression of both the transporter and the dehydrogenase enables\n \n S<\/jats:italic>\n <\/jats:styled-content>\n ynechocystis<\/jats:italic>\n to utilize phosphite, confirming the function of\n T<\/jats:styled-content>\n ery_0365\u20100367 as a phosphite uptake system (\n PtxABC<\/jats:styled-content>\n ) and\n T<\/jats:styled-content>\n ery_0368 as a phosphite dehydrogenase (\n PtxD<\/jats:styled-content>\n ). Our findings suggest that reported uptake of phosphite by\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n consortia in the field likely reflects an active biological process by\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n . These results highlight the diversity of phosphorus sources available to\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richodesmium<\/jats:italic>\n in a resource\u2010limited ocean.\n <\/jats:p>","DOI":"10.1111\/1758-2229.12308","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T02:07:00Z","timestamp":1434506820000},"page":"824-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Phosphite utilization by the globally important marine diazotroph\n \n T<\/i>\n <\/scp>\n richodesmium<\/i>"],"prefix":"10.1111","volume":"7","author":[{"given":"Despo","family":"Polyviou","sequence":"first","affiliation":[{"name":"Ocean and Earth Sciences National Oceanography Centre Southampton University of Southampton Waterfront Campus European Way Southampton SO14\u20093ZH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Hitchcock","sequence":"additional","affiliation":[{"name":"Ocean and Earth Sciences National Oceanography Centre Southampton University of Southampton Waterfront Campus European Way Southampton SO14\u20093ZH UK"},{"name":"Department of Molecular Biology and Biotechnology The University of Sheffield Firth Court Western Bank Sheffield S10\u20092TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison J.","family":"Baylay","sequence":"additional","affiliation":[{"name":"Ocean and Earth Sciences National Oceanography Centre Southampton University of Southampton Waterfront Campus European Way Southampton SO14\u20093ZH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Mark","family":"Moore","sequence":"additional","affiliation":[{"name":"Ocean and Earth Sciences National Oceanography Centre Southampton University of Southampton Waterfront Campus European Way Southampton SO14\u20093ZH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Bibby","sequence":"additional","affiliation":[{"name":"Ocean and Earth Sciences National Oceanography Centre Southampton University of Southampton Waterfront Campus European Way Southampton SO14\u20093ZH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2010.55.4.1768"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20121723"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5316.1221"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04203"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo639"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.149"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.75"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.240"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/3.3.109"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-marine-010213-135046"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo234"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106840108"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04111"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02612.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02550"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo667"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/es020738b"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01968.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408134111"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06187"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.5.1367-1375.1993"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/35075041"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318670111"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8817.2001.037002310.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0775-9"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07659"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.115"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa8181"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1422332112"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2010.55.4.1755"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093357"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5480.759"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.12.004"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00833.x"}],"container-title":["Environmental 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