{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:03:58Z","timestamp":1761897838445,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,8,19]],"date-time":"2015-08-19T00:00:00Z","timestamp":1439942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT) Ci\u00eancia 2007"},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2015,12]]},"abstract":"Summary<\/jats:title>\n \n The first evidence of sexual reproduction in\n \n A<\/jats:italic>\n <\/jats:styled-content>\n spergillus fumigatus<\/jats:italic>\n was reported in 2009. Nevertheless, it remains difficult to understand how\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n is able to reproduce through this mode in its natural environment and how frequently this occurs. The aim of this study was to analyse single nucleotide polymorphisms (\n SNPs<\/jats:styled-content>\n ) in a set of environmental and clinical isolates of\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n to detect signatures of recombination. A group of closely related\n P<\/jats:styled-content>\n ortuguese\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n isolates was characterized by mating type and the genetic diversity of the intergenic regions, microsatellites and multilocus sequence typing (\n MLST<\/jats:styled-content>\n ) genes. A group of 19\n SNPs<\/jats:styled-content>\n , organized in nine association groups and inherited in blocks, was identified and compared. Several variations on the association panel were detected on reference isolates of\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n \u2005\n AF<\/jats:styled-content>\n 293 and\n A<\/jats:styled-content>\n 1163, the sequence types available at\n MLST<\/jats:styled-content>\n database and six clinical and environmental\n P<\/jats:styled-content>\n ortuguese isolates. About one to four haplotype disruptions were observed per isolate. Considering clinical and environmental isolates, sexual reproduction seems to occur more frequently than previously admitted in\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n . In this study, a practical\n SNP<\/jats:styled-content>\n approach is proposed for detection of recombination events in larger collections of\n \n A<\/jats:italic>\n <\/jats:styled-content>\n .\u2009fumigatus<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/1758-2229.12321","type":"journal-article","created":{"date-parts":[[2015,7,17]],"date-time":"2015-07-17T02:23:27Z","timestamp":1437099807000},"page":"881-886","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Recombination detection in\n \n A<\/i>\n <\/scp>\n spergillus fumigatus<\/i>\n through single nucleotide polymorphisms typing"],"prefix":"10.1111","volume":"7","author":[{"given":"Joana","family":"Teixeira","sequence":"first","affiliation":[{"name":"IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto Porto Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto Porto 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