{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:40:13Z","timestamp":1789180813183,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T00:00:00Z","timestamp":1470787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Organohalide respiring bacteria (ORB) are capable of utilising organohalides as electron acceptors for the generation of cellular energy and consequently play an important role in the turnover of natural and anthropogenically\u2010derived organohalides. In this study, the response of a\n                    <jats:italic>Dehalobacter<\/jats:italic>\n                    sp. strain UNSWDHB to the addition of trichloromethane (TCM) after a 50 h period of its absence (suffocation) was evaluated from a transcriptomic and proteomic perspective. The up\u2010regulation of TCM reductive dehalogenase genes (\n                    <jats:italic>tmrABC<\/jats:italic>\n                    ) and their gene products (TmrABC) was confirmed at both transcriptional and proteomic levels. Other findings include the upregulation of various hydrogenases (membrane\u2010associated Ni\u2010Fe hydrogenase complexes and soluble Fe\u2010Fe hydrogenases), formate dehydrogenases, complex I and a pyrophosphate\u2010energized proton pump. The elevated expression of enzymes associated with carbon metabolism, including complete Wood Ljungdahl pathway, during TCM respiration raises interesting questions on possible fates of intracellular formate and its potential role in the physiology of this bacterium. Overall, the findings presented here provide a broader view on the bioenergetics and general physiology of\n                    <jats:italic>Dehalobacter<\/jats:italic>\n                    UNSWDHB cells actively respiring with TCM.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12444","type":"journal-article","created":{"date-parts":[[2016,7,25]],"date-time":"2016-07-25T05:20:21Z","timestamp":1469424021000},"page":"814-824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Genomic, transcriptomic and proteomic analyses of\n                    <i>Dehalobacter<\/i>\n                    UNSWDHB in response to chloroform"],"prefix":"10.1111","volume":"8","author":[{"given":"Bat\u2010Erdene","family":"Jugder","sequence":"first","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haluk","family":"Ertan","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"},{"name":"Department of Molecular Biology and Genetics Istanbul University  Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yie Kuan","family":"Wong","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nady","family":"Braidy","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, School of Psychiatry Centre for Healthy Brain Ageing, University of New South Wales Sydney Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Manefield","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Marquis","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-11-3633"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258118"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4494-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00720-13"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12387"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2005.01.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3866-3871.2005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3ra22668a"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-015-0226-4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2015.07.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00249"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09375"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.3787426"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02370-14"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01070-06"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02656.x"},{"key":"e_1_2_6_18_1","volume-title":"Escherichia coli and Salmonella Typhimurium: Cellular and Molecular Biology","author":"Lin E.","year":"1987"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.006"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-287"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5141-5147.2000"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02130-14"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.072615499"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-011-9447-2"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01090.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0341261"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"4140","DOI":"10.1128\/JB.180.16.4140-4145.1998","article-title":"Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli","volume":"180","author":"Neumann A.","year":"1998","journal-title":"J Bacteriol"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.6.2262-2274.2006"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.051916606"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00069"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00855-06"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0325"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00751"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.8.2328-2333.1996"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01582-08"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00312.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052038"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0318"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050196r"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01042-08"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0317"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00266-16"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1322"},{"key":"e_1_2_6_46_1","doi-asserted-by":"crossref","unstructured":"Wong Y.K. Holland S.I. Ertan H. Manefield M. andLee M.(2016)Isolation and characterization of Dehalobacter sp. strain UNSWDHB capable of chloroform and chlorinated ethane respiration. Environ Microbiol (in press). doi:10.1111\/1462-2920.13287","DOI":"10.1111\/1462-2920.13287"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119507"}],"container-title":["Environmental Microbiology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1758-2229.12444","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1758-2229.12444","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T02:16:04Z","timestamp":1761876964000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1758-2229.12444"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,10]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/1758-2229.12444"],"URL":"https:\/\/doi.org\/10.1111\/1758-2229.12444","archive":["Portico"],"relation":{},"ISSN":["1758-2229","1758-2229"],"issn-type":[{"value":"1758-2229","type":"print"},{"value":"1758-2229","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,10]]},"assertion":[{"value":"2016-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}