{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:46:49Z","timestamp":1790243209290,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T00:00:00Z","timestamp":1498521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology of Japan","doi-asserted-by":"crossref","award":["26252065"],"award-info":[{"award-number":["26252065"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agriculture, Forestry, Fisheries and Food Industry"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In\n                    <jats:italic>Bradyrhizobium diazoefficiens<\/jats:italic>\n                    , maximal expression of the nitrous oxide reductase gene (\n                    <jats:italic>nosZ<\/jats:italic>\n                    ) requires oxygen limitation and the presence of a nitrogen oxide. The putative transcription antiterminator NasT is a positive regulator of\n                    <jats:italic>nosZ<\/jats:italic>\n                    ; but in the absence of nitrate, NasT is counteracted by the nitrate sensor NasS. Here, we examined the NasT\u2010mediated mechanism of\n                    <jats:italic>nosRZDFYLX<\/jats:italic>\n                    gene cluster expression. We mapped two transcription start sites of\n                    <jats:italic>nosR<\/jats:italic>\n                    and identified two potential hairpins, H1 and H2, within the 5\u2032\u2010leader of\n                    <jats:italic>nosR<\/jats:italic>\n                    transcripts. Electrophoretic mobility shift assay showed that NasT specifically bound the\n                    <jats:italic>nosR<\/jats:italic>\n                    \u2010leader RNA and deletion of H1 abolished such binding. Under aerobic nitrate\u2010deficient conditions, deletion of H1 or H2 increased the level of\n                    <jats:italic>nosRZD<\/jats:italic>\n                    transcripts. Under denitrifying conditions (anaerobiosis with nitrate supply), the level of\n                    <jats:italic>nosRZD<\/jats:italic>\n                    transcripts was severely impaired in the\n                    <jats:italic>nasT<\/jats:italic>\n                    mutant; in the\n                    <jats:italic>nasT<\/jats:italic>\n                    background, deletions of either hairpin led to increased level of\n                    <jats:italic>nosRZD<\/jats:italic>\n                    transcripts. In contrast to\n                    <jats:italic>nosRZD<\/jats:italic>\n                    coding region,\n                    <jats:italic>nosR<\/jats:italic>\n                    \u2010leader transcript level was not affected by\n                    <jats:italic>nasS<\/jats:italic>\n                    or\n                    <jats:italic>nasT<\/jats:italic>\n                    mutations under aerobic or denitrifying conditions respectively. These results suggest that the two\u2010hairpin RNA structure acts for transcription termination upstream of\n                    <jats:italic>nosR<\/jats:italic>\n                    and the binding of NasT to H1 facilitates read\u2010through transcription to induce\n                    <jats:italic>nos<\/jats:italic>\n                    expression.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12543","type":"journal-article","created":{"date-parts":[[2017,5,5]],"date-time":"2017-05-05T00:12:39Z","timestamp":1493943159000},"page":"389-396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Regulation of nitrous oxide reductase genes by NasT\u2010mediated transcription antitermination in\n                    <i>Bradyrhizobium diazoefficiens<\/i>"],"prefix":"10.1111","volume":"9","author":[{"given":"Cristina","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University 2\u20101\u20101 Katahira Aoba\u2010Ku Sendai 980\u20108577 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisayuki","family":"Mitsui","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University 2\u20101\u20101 Katahira Aoba\u2010Ku Sendai 980\u20108577 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiwamu","family":"Minamisawa","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University 2\u20101\u20101 Katahira Aoba\u2010Ku Sendai 980\u20108577 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