{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:43:33Z","timestamp":1779331413607,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T00:00:00Z","timestamp":1519344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Physiological constituents in airway surface liquids (ASL) appear to impact the adherence and invasion potentials of\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    contributing to recrudescent melioidosis. Here, we investigated the factors present in ASL that is likely to influence bacterial adhesion and invasion leading to improved understanding of bacterial pathogenesis. Six\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    clinical isolates from different origins were used to investigate the ability of the bacteria to adhere and invade A549 human lung epithelial cells using a system that mimics the physiological ASL with different pH, NaCl, KCl, CaCl\n                    <jats:sub>2<\/jats:sub>\n                    and glucose concentrations. These parameters resulted in markedly differential adherence and invasion abilities of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    to the lung epithelial cells. The concentration of 20 mM glucose dramatically increased adherence and invasion by increasing the rate of pili formation in depiliated bacteria. Glucose significantly increased adherence and invasion of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    to A549 cells, and presence of NaCl, KCl and CaCl\n                    <jats:sub>2<\/jats:sub>\n                    markedly ablated the effect despite the presence of\u00a0glucose. Our data established a link between glucose, enhanced adhesion and invasion potentials of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    , hinting increased susceptibility of individuals with diabetes mellitus to clinical melioidosis.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12624","type":"journal-article","created":{"date-parts":[[2018,2,2]],"date-time":"2018-02-02T06:17:06Z","timestamp":1517552226000},"page":"217-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Adhesion and invasion attributes of\n                    <i>Burkholderia pseudomallei<\/i>\n                    are dependent on airway surface liquid and glucose concentrations in lung epithelial cells"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2351-1597","authenticated-orcid":false,"given":"Vanitha","family":"Mariappan","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaikumar","family":"Thimma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumutha Malar","family":"Vellasamy","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esaki M","family":"Shankar","sequence":"additional","affiliation":[{"name":"Division of Infection Biology and Microbiology, Department of Life Sciences Central University of Tamil Nadu (CUTN) Thiruvarur 610 101 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamuna","family":"Vadivelu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"5692","DOI":"10.1128\/IAI.66.12.5692-5697.1998","article-title":"Environmental growth conditions influence the ability of Escherichia coli K1 to invade brain microvascular endothelial cells and confer serum resistance","volume":"66","author":"Badger J.L.","year":"1998","journal-title":"Infect 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