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Entry into a viable but non\u2010culturable (VBNC) state is a survival strategy by which\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    withstands natural stresses and is important for the transition between the aquatic and host environments during the\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    life cycle. In this study, the formation of VBNC\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    induced by cold seawater exposure was investigated using RNA sequencing (RNA\u2010seq). The analysis revealed that the expression of 1420 genes was changed on VBNC state formation. In the VBNC cells, genes related to biofilm formation, chitin utilization and stress responses were upregulated, whereas those related to cell division, morphology and ribosomal activity were mainly downregulated. The concurrent acquisition of a carbon source and the arrest of cell division in cells with low metabolic activity help bacteria increase their resistance to unfavourable environments. Moreover, two transcriptional regulators, SlmA and MetJ, were found to play roles in both VBNC formation and intestinal colonization, suggesting that some genes may function in both processes. This acquired knowledge will improve our understanding of the molecular mechanisms of stress tolerance and may help control future cholera infections and outbreaks.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12685","type":"journal-article","created":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T03:19:53Z","timestamp":1532920793000},"page":"594-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["RNA\u2010seq\u2010based monitoring of gene expression changes of viable but non\u2010culturable state of\n                    <i>Vibrio cholerae<\/i>\n                    induced by cold seawater"],"prefix":"10.1111","volume":"10","author":[{"given":"Tingting","family":"Xu","sequence":"first","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin People's Republic of China"},{"name":"College of Life Sciences Nankai University  Tianjin People's Republic 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