{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:20:40Z","timestamp":1787800840647,"version":"build-2784847793"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["P08\u2010CVI03591"],"award-info":[{"award-number":["P08\u2010CVI03591"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["BIO2014\u201054361\u2010R"],"award-info":[{"award-number":["BIO2014\u201054361\u2010R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The nitrate\u2010reducing betaproteobacteria\n                    <jats:italic>Azoarcus anaerobius<\/jats:italic>\n                    and\n                    <jats:italic>Thauera aromatica<\/jats:italic>\n                    AR\u20101 use an oxidative mechanism to anaerobically degrade resorcinol and 3,5\u2010dihydroxybenzoate (3,5\u2010DHB), respectively, rendering hydroxyhydroquinone as intermediate. The first pathway step is performed by a dimethylsulphoxide\u2010reductase family hydroxylase. The gene cluster coding for the pathway is homologous in these strains. Only these two\n                    <jats:italic>Rhodocyclales<\/jats:italic>\n                    are known to follow this anaerobic pathway, and nothing is known about its distribution in prokaryotes. To determine the relevance and diversity of this strategy in nature, we enriched for bacteria able to oxidize resorcinol or 3,5\u2010DHB under denitrifying conditions. Nitrate\u2010reducing bacteria able to degrade these compounds were present in soil, aquifer and marine sediments. We were able to isolate a number of strains with this capacity from soil and aquifer samples. Amplicon libraries of\n                    <jats:italic>rehL<\/jats:italic>\n                    , the gene encoding the first step of this pathway, showed an overall low diversity, most sequences clustering with either pathway enzyme. Isolates belonging to the\n                    <jats:italic>Beta\u2010<\/jats:italic>\n                    and\n                    <jats:italic>Gammaproteobacteria<\/jats:italic>\n                    able to grow on these substrates revealed\n                    <jats:italic>rehL<\/jats:italic>\n                    homologues only in strains belonging to\n                    <jats:italic>Thauera<\/jats:italic>\n                    and\n                    <jats:italic>Azoarcus<\/jats:italic>\n                    . Analysis of sequenced genomes in the databases detected the presence of highly similar clusters in two additional betaproteobacteria and in the gammaproteobacterium\n                    <jats:italic>Sedimenticola selenatireducens<\/jats:italic>\n                    , although anaerobic growth on a dihydroxyaromatic could only be confirmed in\n                    <jats:italic>Thauera chlorobenzoica<\/jats:italic>\n                    3CB\u20101. The presence of mobile elements in the flanking sequences of some of the clusters suggested events of horizontal gene transfer, probably contributing to expand the pathway to a broader host range within the\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12752","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T19:22:04Z","timestamp":1552936924000},"page":"525-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Occurrence and diversity of the oxidative hydroxyhydroquinone pathway for the anaerobic degradation of aromatic compounds in nitrate\u2010reducing bacteria"],"prefix":"10.1111","volume":"11","author":[{"given":"Daniel","family":"Pacheco\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Department of Environmental Protection Consejo Superior de Investigaciones Cient\u00edficas, Estaci\u00f3n Experimental del Zaid\u00edn  Profesor Albareda 1, E\u201018008, Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Rama\u2010Garda","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Consejo Superior de Investigaciones Cient\u00edficas, Estaci\u00f3n Experimental del Zaid\u00edn  Profesor Albareda 1, E\u201018008, Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Consejo Superior de Investigaciones Cient\u00edficas, Estaci\u00f3n Experimental del Zaid\u00edn  Profesor Albareda 1, E\u201018008, Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie\u2010Marie","family":"Martirani\u2010Von Abercron","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Consejo Superior de Investigaciones Cient\u00edficas, Estaci\u00f3n Experimental del Zaid\u00edn  Profesor Albareda 1, E\u201018008, Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6289-3348","authenticated-orcid":false,"given":"Silvia","family":"Marqu\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Consejo Superior de Investigaciones Cient\u00edficas, Estaci\u00f3n Experimental del Zaid\u00edn  Profesor Albareda 1, E\u201018008, Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03934-12"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","DOI":"10.1201\/EBK1439804063","volume-title":"Handbook of Mirobiological Media","author":"Atlas R.M.","year":"2010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12328"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2012.07.002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02287-14"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00021-08"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw929"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01729-06"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.91"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2652"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050579"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80023-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00249065"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2013.01.011"},{"key":"e_1_2_6_16_1","volume-title":"Resorcinol","author":"Hahn S.","year":"2006"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.02.027"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00577.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/8.3.275"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15117985"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00384.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja103448u"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07856.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF00264378","article-title":"Holophaga foetida gen. nov., sp. nov., a new, homoacetogenic bacterium degrading methoxylated aromatic compounds","volume":"162","author":"Liesack W.","year":"1994","journal-title":"Arch Microbiol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40793-016-0191-5"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1890\/06-0219"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01353"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01775"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt376"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404378101"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01698-15"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100131"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.12.011"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03042-16"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01392-12"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.14.3644-3649.1998"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002039900112"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2011.00304.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443997"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00004-7"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.7.1628"},{"key":"e_1_2_6_42_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001140050002"},{"key":"e_1_2_6_44_1","volume-title":"Colorimetric Methods of Analysis : Including Some Turbidimetric and Nephelometric Methods","author":"Snell F.D.","year":"1949"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-2-551"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-2-589"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-3-953"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-011-9929-1"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.003329"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20110733"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fny041"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00414460"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky092"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-2191-1_21"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00407804"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30744-3_20"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.036400-0"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.2.316-322.1996"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-2734-4.50017-6"}],"updated-by":[{"DOI":"10.1111\/1758-2229.13026","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000}}],"container-title":["Environmental Microbiology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1758-2229.12752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1758-2229.12752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1758-2229.12752","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1758-2229.12752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T04:50:05Z","timestamp":1761886205000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1758-2229.12752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,27]]},"references-count":59,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1111\/1758-2229.12752"],"URL":"https:\/\/doi.org\/10.1111\/1758-2229.12752","archive":["Portico"],"relation":{},"ISSN":["1758-2229","1758-2229"],"issn-type":[{"value":"1758-2229","type":"print"},{"value":"1758-2229","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,27]]},"assertion":[{"value":"2018-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}