{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:24:50Z","timestamp":1761870290328,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T00:00:00Z","timestamp":1560902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Cryptococcosis is an opportunistic infection caused by the\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    species complex. An outbreak of cryptococcosis caused by\n                    <jats:italic>Cryptococcus gattii<\/jats:italic>\n                    (AFLP6\/VGII) in North America has indicated the need for studies of this organism and its environmental niche. Difficulties in isolating the\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    spp. because of the overgrowth of filamentous fungi onto culture media and its low fungal population size under natural conditions limit studies of these pathogenic yeasts. We designed a selective medium that inhibits the growth of environmental filamentous fungi but does not inhibit that of\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    cells. After enrichment in acidified YPD media and inoculation onto selective media,\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    cells in brown\u2010coloured colonies were isolated from environmental materials. This two\u2010step method is useful for isolating environmental members of the\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    species complex, which is essential for further studies involving diversity and the microbe\u2013environment relationship of this yeast.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12775","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T07:39:21Z","timestamp":1560929961000},"page":"651-658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Two\u2010step method for isolating\n                    <i>Cryptococcus<\/i>\n                    species complex from environmental material using a new selective 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