{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:46:19Z","timestamp":1790232379391,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2020,10]]},"abstract":"Abstract<\/jats:title>\n \n Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended\u2010spectrum beta\u2010lactamase (ESBL)\u2010producing\n Enterobacteriaceae<\/jats:italic>\n , and particularly\n Escherichia coli<\/jats:italic>\n , have spread worldwide as one of the most common multidrug\u2010resistant organisms. The aim of this study was to determine the carriage rate of ESBL\u2010producing\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free\u2010ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2\u2009\u03bcg\u2009ml\n \u22121<\/jats:sup>\n ). Colonies with typical morphology of\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n were identified by MALDI\u2010TOF\u2010MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double\u2010disk tests. The presence of\n bla<\/jats:italic>\n CTX\u2010M<\/jats:sub>\n ,\n bla<\/jats:italic>\n SHV<\/jats:sub>\n ,\n bla<\/jats:italic>\n TEM<\/jats:sub>\n ,\n bla<\/jats:italic>\n KPC<\/jats:sub>\n and\n bla<\/jats:italic>\n OXA\u201048<\/jats:sub>\n genes, as well as\n mcr\u2010<\/jats:italic>\n 1 (colistin resistance),\n tet<\/jats:italic>\n A\/\n tet<\/jats:italic>\n B and\n int<\/jats:italic>\n 1 gene, was tested by PCR\/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR\/sequencing. ESBL\u2010producing\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n isolates were detected in 5\/22 tested animals (22.7%), and all isolates (one\/animal) carried\n bla<\/jats:italic>\n CTX\u2010M<\/jats:sub>\n genes:\n bla<\/jats:italic>\n CTX\u2010M\u201015<\/jats:sub>\n (\n n<\/jats:italic>\n =\u20093) and\n bla<\/jats:italic>\n CTX\u2010M\u201055<\/jats:sub>\n (\n n<\/jats:italic>\n =\u20092). ESBL\u2010positive isolates were ascribed to phylogenetic group D (two isolates), B\n 1<\/jats:sub>\n (two isolates) and A (one isolate), and five sequence types were detected (ST\/phylogenetic\u2010group\/ESBL): ST515\/B1\/CTX\u2010M\u201015, ST1290\/A\/CTX\u2010M\u201015, ST38\/D\/CTX\u2010M\u201015, ST457\/D\/CTX\u2010M\u201055 and ST6448\/B\n 1<\/jats:sub>\n \/CTX\u2010M\u201055; this suggests a genetic diversity among these isolates. Three CTX\u2010M\u201015\u2010producing isolates contained the\n bla<\/jats:italic>\n TEM<\/jats:sub>\n gene and one the\n tet<\/jats:italic>\n A gene. To our knowledge, this appears to be the first report of ESBL\u2010producing\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n in vulture chicks from the Canary Islands.\n <\/jats:p>","DOI":"10.1111\/1758-2229.12873","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T22:02:26Z","timestamp":1596578546000},"page":"540-547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Molecular diversity of\n Extended\u2010spectrum<\/scp>\n \u03b2\u2010lactamase\u2010producing\n \n Escherichia coli<\/i>\n <\/scp>\n from vultures in Canary Islands"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-412X","authenticated-orcid":false,"given":"Isabel","family":"Carvalho","sequence":"first","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila 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University of Las Palmas de Gran Canaria Canary Islands Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Silva","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila Real Portugal"},{"name":"Department of Genetics and Biotechnology University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila Real Portugal"},{"name":"Functional Genomics and Proteomics Unit University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila Real Portugal"},{"name":"Laboratory Associated for Green Chemistry (LAQV\u2010REQUIMTE) New University of Lisbon Monte da Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila 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