{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:58:01Z","timestamp":1761897481309,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,7]],"date-time":"2021-02-07T00:00:00Z","timestamp":1612656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Two global cycles, iron and sulfur, are critically interconnected in estuarine environments by microbiological actors. To this point, the methods of laboratory study of this interaction have been limited. Here we propose a methodology for co\u2010culturing from numerous coastal environments, from the same source inocula, iron\u2010oxidizing and sulfate\u2010reducing bacteria. The use of same source inocula is largely beneficial to understand real\u2010world interactions that are likely occurring\n                    <jats:italic>in situ<\/jats:italic>\n                    . Through the use of this methodology, the ecological interactions between these groups can be studied in a more controlled environment. Here, we characterize the oxygen and hydrogen sulfide concentrations using microelectrode depth profiling in the co\u2010cultures of iron\u2010oxidizing bacteria and sulfate\u2010reducing bacteria. These results suggest that while oxygen drives the relationship between these organisms and sulfate\u2010reducers are reliant on iron\u2010oxidizers in this culture to create an anoxic environment, there is likely another environmental driver that also influences the interaction as the two remain spatially distinct, as trends in FeS precipitation changed within the anoxic zone relative to the presence of Fe(III) oxyhydroxides. Understanding the relationship between iron\u2010oxidizing and sulfate\u2010reducing bacteria will ultimately have implications for understanding microbial cycling in estuarine environments as well as in processes such as controlling microbially influenced corrosion.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.12932","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T15:04:49Z","timestamp":1612796689000},"page":"317-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Orange leads to black: evaluating the efficacy of co\u2010culturing iron\u2010oxidizing and sulfate\u2010reducing bacteria to discern ecological relationships"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7684-6470","authenticated-orcid":false,"given":"Chequita N.","family":"Brooks","sequence":"first","affiliation":[{"name":"Department of Biology East Carolina 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