{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:40:12Z","timestamp":1791312012476,"version":"4.3.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The genome of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    O139 strains has undergone cryptic changes since its first emergence in 1992 in South India. This study aimed to determine the presence of genotypic changes marked in\n                    <jats:italic>ctx<\/jats:italic>\n                    B,\n                    <jats:italic>tcp<\/jats:italic>\n                    A and\n                    <jats:italic>rst<\/jats:italic>\n                    R genes located within the CTX prophages among the strains of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 isolated from 1999 to 2017 in Odisha. Antibiotic susceptibility test was conducted on 59\u2009\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 strains. A conventional PCR assay was done for\n                    <jats:italic>ctx<\/jats:italic>\n                    B gene typing followed by sequencing along with identification of\n                    <jats:italic>rst<\/jats:italic>\n                    R and\n                    <jats:italic>tcp<\/jats:italic>\n                    A gene. Pulsed\u2010field gel electrophoresis (PFGE) was carried out to reveal clonal variations among the\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 strains. Among\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 isolates more than 60% showed resistance to ampicillin, co\u2010trimoxazole, furazolidone, streptomycin, neomycin and nalidixic acid. The\n                    <jats:italic>ctx<\/jats:italic>\n                    B sequencing and\n                    <jats:italic>rst<\/jats:italic>\n                    R allele\u2010specific PCR assay revealed the presence of three genotypes 1, 3 and 4 with at least one copy of CTX Calc \u03c6 in addition to CTX ET and CTX Cl prophages in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 isolates. PFGE analysis revealed 13 pulsotypes with two clades having 60% similarity among\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 strains. The circulating\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139 strains in Odisha showed variation in genotypes with multiple clonal expansions over the years.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.13016","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T07:36:30Z","timestamp":1634801790000},"page":"376-384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genomic diversities of\n                    <scp>\n                      <i>ctx<\/i>\n                      B\n                    <\/scp>\n                    ,\n                    <scp>\n                      <i>tcp<\/i>\n                      A\n                    <\/scp>\n                    and\n                    <scp>\n                      <i>rst<\/i>\n                      R\n                    <\/scp>\n                    alleles of\n                    <i>Vibrio cholerae<\/i>\n                    \u2009\n                    <scp>O139<\/scp>\n                    strains isolated from Odisha, India"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7605-3342","authenticated-orcid":false,"given":"Dipti Ranjan","family":"Behera","sequence":"first","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-8820","authenticated-orcid":false,"given":"Ashish Kumar","family":"Nayak","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2807-6037","authenticated-orcid":false,"given":"Smruti Ranjan","family":"Nayak","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilena","family":"Nayak","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sipraswati","family":"Swain","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep Kumar","family":"Maharana","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5019-2325","authenticated-orcid":false,"given":"Bhagyalaxmi","family":"Biswal","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4146-6064","authenticated-orcid":false,"given":"Swatishree","family":"Pany","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-5592","authenticated-orcid":false,"given":"Sanghamitra","family":"Pati","sequence":"additional","affiliation":[{"name":"Microbiology Division ICMR\u2010Regional Medical Research Centre  Chandrasekharpur, Bhubaneswar OR 751023 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