{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:47:39Z","timestamp":1787597259473,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201001672\u201013"],"award-info":[{"award-number":["APQ\u201001672\u201013"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate whether penicillin\u2010resistant, ampicillin\u2010susceptible\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    (PRASEF) isolates are disseminated in non\u2010clinical sources, and to compare the molecular characteristics and antimicrobial resistance (AMR) profile of clinical and non\u2010clinical\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    isolates. Non\u2010clinical samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u2009280) were collected and 101\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    isolates were recovered from food (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200918), faeces of healthy animals (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200924), water (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200928) and sewage (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200931). PRASEF (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200968) and penicillin\u2010susceptible, ampicillin\u2010susceptible\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200977) isolates of clinical origin were also evaluated. A significant variety of AMR profiles was observed among non\u2010clinical isolates according to the source. No food isolate exhibited a multidrug resistance (MDR) phenotype different from those of isolates from animal faeces (50.0%) and sewage (38.7%). Overall, the MDR phenotype was more frequent among clinical (56.6%) than non\u2010clinical isolates (22.8%) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u20090.01). Non\u2010clinical PRASEF isolates (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u20093) were only recovered from hospital sewage. Note that representative clinical and non\u2010clinical PRASEF isolates were grouped in pulsotype A, and belonged to CC9 (clonal complex). In conclusion,\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    isolates exhibiting the unusual penicillin\u2010resistant but ampicillin\u2010susceptible phenotype appeared to be restricted to the hospital environment. Our findings highlight the ability of PRASEF isolates to survive in sewage, which could enable these hospital\u2010adapted lineages to spread to new ecological niches.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.13043","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T04:42:10Z","timestamp":1642740130000},"page":"230-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Penicillin\u2010resistant, ampicillin\u2010susceptible\n                    <i>Enterococcus faecalis<\/i>\n                    isolates are uncommon in non\u2010clinical sources"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9995-3746","authenticated-orcid":false,"given":"F\u00e1bio Ederson Lopes","family":"Correa","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas e Naturais Universidade Federal do Tri\u00e2ngulo Mineiro  Uberaba Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemeire Cobo","family":"Zanella","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Cassiolato","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Dias","family":"Paiva","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas e Naturais Universidade Federal do Tri\u00e2ngulo Mineiro  Uberaba Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica Hitomi","family":"Okura","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Tecnol\u00f3gicas e Exatas Universidade Federal do Tri\u00e2ngulo Mineiro  Uberaba Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas e Naturais Universidade Federal do Tri\u00e2ngulo Mineiro  Uberaba Minas Gerais Brazil"},{"name":"Instituto Federal de Educa\u00e7\u00e3o Ci\u00eancia e Tecnologia de Rond\u00f4nia  Colorado do Oeste Rond\u00f4nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Gon\u00e7alves","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas e Naturais Universidade Federal do Tri\u00e2ngulo Mineiro  Uberaba Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.11.067"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132586"},{"key":"e_1_2_7_4_1","volume-title":"Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet]","author":"Agudelo Higuita N.I.","year":"2014"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-018-01784-z"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2008.05.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7264"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.01.020"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2018.11.012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822014000100016"},{"key":"e_1_2_7_11_1","unstructured":"CLSI. 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