{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:26:25Z","timestamp":1787495185638,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91951206"],"award-info":[{"award-number":["91951206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41630857"],"award-info":[{"award-number":["41630857"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Application of ferric iron is conventionally considered to inhibit methanogenesis in anoxic environments. Here we show that\n                    <jats:italic>Methanosarcina mazei<\/jats:italic>\n                    zm\u201015, a strain isolated from the natural wetland of Tibetan plateau, is capable of Fe(III) reduction, which significantly promotes its growth and methanogenesis. We grew\n                    <jats:italic>Ms<\/jats:italic>\n                    .\n                    <jats:italic>mazei<\/jats:italic>\n                    zm\u201015 in a medium containing acetate supplemented with Fe(III) in ferric citrate or ferrihydrite and to some cultures anthraquinone\u20102,6\u2010disulfonate (AQDS) was applied as an electron shuttle. The reduction of Fe(III) species occurred immediately. Ferric citrate was more readily reduced than ferrihydrite. The X\u2010ray diffraction spectra analysis showed the formation of magnetite from ferrihydrite and amorphous reduced products from ferrihydrite plus AQDS. The analysis of protein contents revealed that Fe(III) reduction contributed 36%\u201346% of the cell growth. The growth yield, estimated as protein increment per acetate consumed for Fe(III) reduction, increased by 20\u2010 to 30\u2010fold compared with methanogenesis, which is in consistence with the difference in free energy available by Fe(III) reduction relative to methanogenesis. We propose that the outer\u2010surface multiheme c\u2010type cytochrome predicted from\n                    <jats:italic>Ms<\/jats:italic>\n                    .\n                    <jats:italic>mazei<\/jats:italic>\n                    zm\u201015 genome serves as the terminal reductase with the energy\u2010converting hydrogenase and F\n                    <jats:sub>420<\/jats:sub>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    dehydrogenase involved in electron transport chain for Fe(III) reduction. The findings shed a light to better understand the ecophysiology of\n                    <jats:italic>Methanosarcina<\/jats:italic>\n                    in anaerobic environments.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.13098","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T21:40:12Z","timestamp":1654033212000},"page":"804-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Coupling methanogenesis with iron reduction by acetotrophic\n                    <i>Methanosarcina mazei<\/i>\n                    zm\u201015"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7282-9190","authenticated-orcid":false,"given":"Zhen","family":"Yang","sequence":"first","affiliation":[{"name":"College of Urban and Environmental Science Peking University  Beijing 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