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The inhibition of the EPSP synthase depletes the cell of the EPSP\u2010derived aromatic amino acids as well as of folate and quinones. A variety of mechanisms (e.g., EPSP synthase modification) has been described that confer GS resistance to bacteria. Here, we show that the\n                    <jats:italic>Burkholderia anthina<\/jats:italic>\n                    strain DSM 16086 quickly evolves GS resistance by the acquisition of mutations in the\n                    <jats:italic>ppsR<\/jats:italic>\n                    gene.\n                    <jats:italic>ppsR<\/jats:italic>\n                    codes for the pyruvate\/ortho\u2010P\n                    <jats:sub>i<\/jats:sub>\n                    dikinase PpsR that physically interacts and regulates the activity of the PEP synthetase PpsA. The mutational inactivation of\n                    <jats:italic>ppsR<\/jats:italic>\n                    causes an increase in the cellular PEP concentration, thereby abolishing the inhibition of the EPSP synthase by GS that competes with PEP for binding to the enzyme. Since the overexpression of the\n                    <jats:italic>Escherichia coli ppsA<\/jats:italic>\n                    gene in\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    did not increase GS resistance in these organisms, the mutational inactivation of the\n                    <jats:italic>ppsR<\/jats:italic>\n                    gene resulting in PpsA overactivity is a GS resistance mechanism that is probably unique to\n                    <jats:italic>B. anthina<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.13184","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T22:13:09Z","timestamp":1686694389000},"page":"727-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genomic adaptation of\n                    <i>Burkholderia anthina<\/i>\n                    to glyphosate uncovers a novel herbicide resistance mechanism"],"prefix":"10.1111","volume":"15","author":[{"given":"Inge","family":"Schwedt","sequence":"first","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"},{"name":"FG Molecular Microbiology, Institute of Biology University of Hohenheim  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline","family":"Collignon","sequence":"additional","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Mittelst\u00e4dt","sequence":"additional","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Giudici","sequence":"additional","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Rapp","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Microbiology and Infection Medicine T\u00fcbingen University of T\u00fcbingen, Bacterial Metabolomics  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janek","family":"Mei\u00dfner","sequence":"additional","affiliation":[{"name":"Department of General Microbiology, Institute for Microbiology and Genetics University of Goettingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannes","family":"Link","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Microbiology and Infection Medicine T\u00fcbingen University of T\u00fcbingen, Bacterial Metabolomics  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Hertel","sequence":"additional","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"},{"name":"Department of Genomic and Applied Microbiology, Institute for Microbiology and Genetics University of Goettingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0323-7479","authenticated-orcid":false,"given":"Fabian M.","family":"Commichau","sequence":"additional","affiliation":[{"name":"FG Synthetic Microbiology, Institute for Biotechnology BTU Cottbus\u2010Senftenberg  Senftenberg Germany"},{"name":"FG Molecular Microbiology, Institute of Biology University of Hohenheim  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Andrews S.(2010)FastQC: a quality control tool for high throughput sequence data. 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