{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:46:35Z","timestamp":1792457195064,"version":"4.5.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T00:00:00Z","timestamp":1719100800000},"content-version":"am","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T00:00:00Z","timestamp":1719100800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019\u201067020\u201030475"],"award-info":[{"award-number":["2019\u201067020\u201030475"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011498","name":"New Jersey Water Resources Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011498","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000146","name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","doi-asserted-by":"publisher","award":["1903597"],"award-info":[{"award-number":["1903597"]}],"id":[{"id":"10.13039\/100000146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005891","name":"Hudson River Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005891","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2024,6]]},"abstract":"Abstract<\/jats:title>\n \n Chryseobacteria<\/jats:italic>\n consists of important human pathogens that can cause a myriad of nosocomial infections. We isolated four multidrug\u2010resistant\n Chryseobacterium<\/jats:italic>\n bacteria from activated sludge collected at domestic wastewater treatment facilities in the New York Metropolitan area. Their genomes were sequenced with Nanopore technology and used for a comprehensive resistomics comparison with 211\n Chryseobacterium<\/jats:italic>\n genomes available in the public databases. A majority of\n Chryseobacteria<\/jats:italic>\n harbor 3 or more antibiotic resistance genes (ARGs) with the potential to confer resistance to at least two types of commonly prescribed antimicrobials. The most abundant ARGs, including \u03b2\u2010lactam class A (\n blaCGA\u20101<\/jats:italic>\n and\n blaCIA<\/jats:italic>\n ) and class B (\n blaCGB\u20101<\/jats:italic>\n and\n blaIND<\/jats:italic>\n ) and aminoglycoside (\n ranA<\/jats:italic>\n and\n ranB<\/jats:italic>\n ), are considered potentially intrinsic in\n Chryseobacteria<\/jats:italic>\n . Notably, we reported a new resistance cluster consisting of a chloramphenicol acetyltransferase gene\n catB11<\/jats:italic>\n , a tetracycline resistance gene\n tetX<\/jats:italic>\n , and two mobile genetic elements (MGEs),\n IS91<\/jats:italic>\n family transposase and\n XerD<\/jats:italic>\n recombinase. Both\n catB11<\/jats:italic>\n and\n tetX<\/jats:italic>\n are statistically enriched in clinical isolates as compared to those with environmental origins. In addition, two other ARGs encoding aminoglycoside adenylyltransferase (\n aadS<\/jats:italic>\n ) and the small multidrug resistance pump (\n abeS<\/jats:italic>\n ), respectively, are found co\u2010located with MGEs encoding recombinases (e.g.,\n RecA<\/jats:italic>\n and\n XerD<\/jats:italic>\n ) or transposases, suggesting their high transmissibility among\n Chryseobacteria<\/jats:italic>\n and across the\n Bacteroidota<\/jats:italic>\n phylum, particularly those with high pathogenicity. High resistance to different classes of \u03b2\u2010lactam, as well as other commonly used antimicrobials (i.e., kanamycin, gentamicin, and chloramphenicol), was confirmed and assessed using our isolates to determine their minimum inhibitory concentrations. Collectively, though the majority of ARGs in\n Chryseobacteria<\/jats:italic>\n are intrinsic, the discovery of a new resistance cluster and the co\u2010existence of several ARGs and MGEs corroborate interspecies and intergenera transfer, which may accelerate their dissemination in clinical environments and complicate efforts to combat bacterial infections.\n <\/jats:p>","DOI":"10.1111\/1758-2229.13288","type":"journal-article","created":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T19:14:05Z","timestamp":1719170045000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Comparative resistomics analysis of multidrug\u2010resistant\n Chryseobacteria<\/i>"],"prefix":"10.1111","volume":"16","author":[{"given":"Dung Ngoc","family":"Pham","sequence":"first","affiliation":[{"name":"Department of Chemistry and Environmental Science New Jersey Institute of Technology Newark New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7664-4836","authenticated-orcid":false,"given":"Mengyan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Environmental Science New Jersey Institute of Technology Newark New Jersey 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