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This study examined physicochemical parameters, chemical contaminants and antibiotic\u2010resistant bacteria in waste stabilization pond effluents, hospital wastewater and domestic wastewater from four sewerage sites in Kumasi. The bacteria isolates were sequenced. Three sites exceeded national guidelines for total suspended solids, biochemical oxygen demand, chemical oxygen demand and electrical conductivity. Although sulfamethoxazole levels were low, the antibiotic was detected at all sites. Multi\u2010drug\u2010resistant\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa were isolated<\/jats:italic>\n                    with multi\u2010locus sequence typing identifying\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains as ST18 and ST147, and\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    as ST235, all of clinical relevance. A comparison of ST147 genomes with isolates from human infections in Africa showed remarkable similarity and shared AMR profiles. Thirteen of the twenty\u2010one plasmids from ST147 harbored at least one AMR gene, including blaCTX\u2010M\u201015 linked to copper\u2010resistance genes. Our study demonstrated high bacterial counts and organic matter in the analysed wastewater. The recovery of clinically significant isolates with multiple antibiotic and heavy metal resistance genes from the wastewater samples raises public health concerns.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70018","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T23:09:14Z","timestamp":1731107354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Recovery of clinically relevant multidrug\u2010resistant\n                    <i>Klebsiella pneumoniae<\/i>\n                    lineages from wastewater in Kumasi Metropolis, Ghana"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2033-8810","authenticated-orcid":false,"given":"Amen","family":"Ekhosuehi","sequence":"first","affiliation":[{"name":"Regional Water and Environmental Sanitation Centre, Kumasi Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3738-4584","authenticated-orcid":false,"given":"Odion O.","family":"Ikhimiukor","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"},{"name":"Department of Biological Sciences University at Albany, State University of New York  Albany New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen Michelle Korkor","family":"Essandoh","sequence":"additional","affiliation":[{"name":"Regional Water and Environmental Sanitation Centre, Kumasi Kwame Nkrumah University of Science and Technology  Kumasi Ghana"},{"name":"Department of Civil Engineering Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana Yaw","family":"Asiedu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isoken Tito","family":"Aighewi","sequence":"additional","affiliation":[{"name":"Regional Water and Environmental Sanitation Centre, Kumasi Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Temitope","family":"Sunmonu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erkison Ewomazino","family":"Odih","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson O.","family":"Oaikhena","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Cyril\u2010Okoh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Yeboah","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1694-7587","authenticated-orcid":false,"given":"Iruka N.","family":"Okeke","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy University of Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab769"},{"key":"e_1_2_10_3_1","volume-title":"Standard methods for the examination of water and wastewater","author":"American Public Health Association; 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