{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:27:44Z","timestamp":1791872864201,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["Synergy Grant 2021\/GNT2010716 (STARFISH)"],"award-info":[{"award-number":["Synergy Grant 2021\/GNT2010716 (STARFISH)"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Improving our understanding of superficial\n                    <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    (Strep A) carriage and transmission necessitates robust sampling methods. Here, we compared the effect of storing swab samples in fridge (+4\u00b0C) and freezer (\u221220\u00b0C) conditions on the recovery of laboratory\u2010cultured\n                    <jats:italic>S. pyogenes<\/jats:italic>\n                    .\n                    <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    colony\u2010forming units progressively declined at +4\u00b0C, but not at \u221220\u00b0C, over 2\u2009weeks. Results demonstrate that freezing is preferable over refrigeration for storage and transport of skin and throat swabs to ensure that culturing accurately reflects the true results of sampling. This is particularly important in remote community research and practice when immediate incubation is not possible or batch processing is most practical, increasing the elapsed time between collection and laboratory analysis. The study suggests that temperature negatively affects\n                    <jats:italic>S. pyogenes<\/jats:italic>\n                    viability and provides a method to further investigate the role of other environmental factors affecting\n                    <jats:italic>S. pyogenes<\/jats:italic>\n                    transmission.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70036","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T23:34:53Z","timestamp":1731108893000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Recovery of culturable\n                    <i>Streptococcus pyogenes<\/i>\n                    from swabs stored at different temperatures"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9013-1244","authenticated-orcid":false,"given":"Kate","family":"Summer","sequence":"first","affiliation":[{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute University of Western Australia  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven Y. C.","family":"Tong","sequence":"additional","affiliation":[{"name":"Victorian Infectious Disease Service, The Peter Doherty Institute for Infection and Immunity, The Royal Melbourne Hospital  Melbourne Victoria Australia"},{"name":"Department of Infectious Diseases, The Peter Doherty Institute for Infection and Immunity The University of Melbourne  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R.","family":"Carapetis","sequence":"additional","affiliation":[{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute University of Western Australia  Nedlands Western Australia Australia"},{"name":"Department of Infectious Diseases Perth Children's Hospital  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha C.","family":"Bowen","sequence":"additional","affiliation":[{"name":"Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute University of Western Australia  Nedlands Western Australia Australia"},{"name":"Department of Infectious Diseases Perth Children's Hospital  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-014-0727-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00865-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v080.i01"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.84"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.3.470"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc09d02s30"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.122.025284"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12913-017-2747-5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00068-X"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268807008023"},{"issue":"5","key":"e_1_2_10_12_1","article-title":"Complete genome assembly of Streptococcus pyogenes ATCC 19615, a Group A \u03b2\u2010hemolytic reference strain","volume":"2","author":"Minogue T.D.","year":"2014","journal-title":"Genome Announcements"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006335"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.20-0341"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph191610215"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.35.2.223"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13692.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1256-8"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0288016"},{"issue":"10","key":"e_1_2_10_20_1","first-page":"S3","article-title":"Ending rheumatic heart disease in Australia: the evidence for a new approach","volume":"213","author":"Wyber R.","year":"2020","journal-title":"Medical Journal of Australia"}],"container-title":["Environmental Microbiology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1758-2229.70036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T01:29:07Z","timestamp":1761874147000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1758-2229.70036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,8]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/1758-2229.70036"],"URL":"https:\/\/doi.org\/10.1111\/1758-2229.70036","archive":["Portico"],"relation":{},"ISSN":["1758-2229","1758-2229"],"issn-type":[{"value":"1758-2229","type":"print"},{"value":"1758-2229","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,8]]},"assertion":[{"value":"2024-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70036"}}