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Identifying these bacteria in hospital environments is crucial for improving healthcare safety. This study aimed to analyse environmental samples from a veterinary hospital to identify prevalent microorganisms and detect antimicrobial resistance patterns. A total of 183 surface samples were collected from 26 areas at the Veterinary Clinical Hospital of Alfonso X el Sabio University in Madrid. The isolated strains were identified, and susceptibility profiles were determined via the disk diffusion method. Clonality analysis was performed using pulsed\u2010field gel electrophoresis. In total, 109 strains were isolated: 76.15% from the\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    family and 23.85% non\u2010fermenting Gram\u2010negative bacilli. The isolates included\n                    <jats:italic>Klebsiella, Enterobacter, Escherichia<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species, which could include high\u2010risk clones, given their ability to carry several antimicrobial resistance genes. The equine area had the highest number of isolates (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200971), accounting for 65% of the total. High resistance indices were observed against at least five of the 16 antibiotics tested, indicating significant multidrug resistance. Clonality analysis suggested potential cross\u2010transmission within the facility. This study sampled hospital surfaces but not personnel or animals, making contamination sources unclear. Without resampling, the effectiveness of cleaning protocols remains uncertain. Results suggest that hospital staff play a key role in bacterial transmission. The lack of specialised preventive measures in veterinary hospitals highlights a need for further research and improvement.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70055","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T11:19:01Z","timestamp":1734952741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence, Distribution and Antimicrobial Susceptibility of\n                    <i>Enterobacteriaceae<\/i>\n                    and Non\u2010Fermenting Gram\u2010Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain"],"prefix":"10.1111","volume":"16","author":[{"given":"Jes\u00fas Antonio","family":"P\u00e9rez Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Facultad de Ciencias de la Salud Universidad Alfonso X el Sabio  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9199-7341","authenticated-orcid":false,"given":"Silvia","family":"Penelo Hidalgo","sequence":"additional","affiliation":[{"name":"Servicio de Urgencias, Hospitalizaci\u00f3n y UCI, Hospital Cl\u00ednico Veterinario Complutense Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8825-1696","authenticated-orcid":false,"given":"Mar\u00eda\u2010Rosario","family":"Baquero Artigao","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud Universidad Alfonso X el Sabio  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8242-2658","authenticated-orcid":false,"given":"Gustavo","family":"Ortiz\u2010D\u00edez","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Veterinario Complutense Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1195-5078","authenticated-orcid":false,"given":"Tania","family":"Ayll\u00f3n Santiago","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud Universidad Alfonso X el Sabio  Madrid Spain"},{"name":"Departamento de Gen\u00e9tica, Fisiolog\u00eda y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas Universidad Complutense  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,23]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"430","article-title":"Prevelance and Antibiotic Susceptibility Patterns of Pantoea Spp. Isolated Form Clinical and Environmental Sources in Iraq","volume":"9","author":"Abdalhussen L. 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