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Direct\u2010infusion high\u2010resolution mass spectrometry (DI\u2010HRMS) was performed on methanol extracts obtained from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P.\u2009infestans<\/jats:italic>\n                    <\/jats:styled-content>\n                    cultivated on needles and malt extract media. DI\u2010HRMS analysis identified 21 compounds from the malt extract and 112 from the needle samples. The resin components increased in the needle samples post\u2010cultivation, suggesting terpenoid release from resin ducts due to fungal degradation of plant cell walls.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P.\u2009infestans<\/jats:italic>\n                    <\/jats:styled-content>\n                    fully consumed sugars and antifungal compounds, including taxiresinol and salicylic acid, with control\u2010to\u2010sample ratios (CTR\/SA) of 289.76 and 47.24, respectively. Moreover, lariciresinol and pinoresinol were reduced to undetectable levels. The genomic analysis identified 421 secreted proteins, including 128 carbohydrate\u2010active enzymes, 3 cutinases, and 49 lipases that aid host penetration and wax degradation. Several multi\u2010drug efflux pumps and two acyclic terpene utilisation proteins were identified as well. These proteins support the cellular integrity of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P.\u2009infestans<\/jats:italic>\n                    <\/jats:styled-content>\n                    by expelling toxic compounds. Our findings provide valuable insights into the metabolic strategies of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P.\u2009infestans<\/jats:italic>\n                    <\/jats:styled-content>\n                    for nutrient assimilation on pine needles.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70084","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:54:56Z","timestamp":1749516896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomic and Genomic Analysis of Bioactive Compounds of\n                    <i>Phacidium infestans<\/i>\n                    Karsten DSM 5139 Cultivated on\n                    <i>Pinus sylvestris<\/i>\n                    Needles"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3285-5818","authenticated-orcid":false,"given":"Chahira","family":"Zerouki","sequence":"first","affiliation":[{"name":"School of Forest Sciences University of Eastern Finland  Joensuu Finland"},{"name":"Department of Environmental and Biological Sciences University of Eastern Finland  Joensuu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omolara","family":"Mofikoya","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Sustainable Technology University of Eastern Finland  Joensuu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taskeen","family":"Badar","sequence":"additional","affiliation":[{"name":"School of Forest Sciences University of Eastern Finland  Joensuu 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