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Under hydrothermal conditions, these alkanes can also be oxidised to their respective alcohols and ketones, with a preference for the 2\u2010position. The thermoacidophilic verrucomicrobial methanotroph\n                    <jats:italic>Methylacidiphilum fumariolicum<\/jats:italic>\n                    SolV, isolated from the Solfatara volcano, was previously shown to oxidise methane as well as the short\u2010chain hydrocarbons propane and ethane. Here, we show the growth of strain SolV on the C3 compounds 2\u2010propanol and acetone with growth rates of 0.054\u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 0.042\u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. In contrast to methanotrophic growth (rate 0.07\u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), growth was not dependent on CO\n                    <jats:sub>2<\/jats:sub>\n                    or lanthanides. Respiration experiments on steady\u2010state continuous cultures showed an apparent affinity of 0.4\u2009\u03bcM acetone and 5.4\u2009\u03bcM 2\u2010propanol. Transcriptomic analysis of these cultures showed that a gene cluster including a novel acetone monooxygenase (PMO3), previously identified in the closely related species\n                    <jats:italic>Methylacidiphilum caldifontis<\/jats:italic>\n                    , was highly upregulated under growth on C3 substrates. These results support the versatile metabolism of verrucomicrobial methanotrophs. The conversion of other compounds besides methane can be important in view of the ecological relevance of methanotrophs.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70129","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T06:49:21Z","timestamp":1751957361000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>C3<\/scp>\n                    Compound Metabolism in the Thermoacidophilic Methanotroph\n                    <i>Methylacidiphilum fumariolicum<\/i>\n                    <scp>SolV<\/scp>"],"prefix":"10.1111","volume":"17","author":[{"given":"Changqing","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8950-7843","authenticated-orcid":false,"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-8334","authenticated-orcid":false,"given":"Stijn","family":"Peeters","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5145-991X","authenticated-orcid":false,"given":"Rob A.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"},{"name":"Department of Biotechnology Delft University of Technology  Delft the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo A.","family":"van Alen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2197-136X","authenticated-orcid":false,"given":"Lena J.","family":"Daumann","sequence":"additional","affiliation":[{"name":"Chair of Bioinorganic Chemistry Heinrich\u2010Heine Universit\u00e4t  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1990-9030","authenticated-orcid":false,"given":"Huub J. M.","family":"Op den Camp","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5418-2741","authenticated-orcid":false,"given":"Wouter","family":"Versantvoort","sequence":"additional","affiliation":[{"name":"Chair of Bioinorganic Chemistry Heinrich\u2010Heine Universit\u00e4t  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Andrews S.2010.\u201cFastQC: A Quality Control Tool for High Troughput Sequence 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