{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:37:01Z","timestamp":1788284221160,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["GURDT I2016\u20101731\u20100006632"],"award-info":[{"award-number":["GURDT I2016\u20101731\u20100006632"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010081","name":"Conseil R\u00e9gional de La R\u00e9union","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extended\u2010spectrum beta\u2010lactamases (ESBL)\u2010producing enterobacterales are considered a key indicator for antimicrobial resistance (AMR) epidemiological surveillance in animal, human, and environment compartments. In this study, we aim to investigate the presence and genetic diversity of ESBL\u2010producing enterobacterales on vegetable plants. We isolated beta\u2010lactam resistant enterobacterales from several vegetable plants and sequenced their whole genome. Utilising standard genomic and phylogenetic methods, we sought to (i) characterise the resistance genes and plasmid content of the plant\u2010isolated strains, (ii) investigate their genetic structure, and (iii) determine their relationships with strains from other reservoirs. Among the 22 strains collected from vegetable plants, 6 showed resistance to beta\u2010lactam antibiotics, with 5 of them identified as ESBL producers. Our results indicated the presence of multidrug\u2010resistant (MDR) strains containing multiple antibiotic resistance genes (ARGs). Importantly, no host\u2010specific lineages were identified among the plant\u2010isolated ESBL\u2010producing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (ESBL\u2010Ec). Instead, these strains exhibited genetic and epidemiological connections with strains isolated from animals, humans, and the environment, suggesting transfer of ESBL\u2010Ec between plants and other sources in rural Madagascar. These preliminary findings suggest that vegetable plants are contaminated as a result of human activities, posing a potential risk of human and animal exposure to antibiotic\u2010resistant bacteria and genes.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70130","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T09:05:31Z","timestamp":1750842331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of\n                    <scp>ESBL<\/scp>\n                    \u2010Producing Enterobacterales From Vegetable Plants: Preliminary Findings From a Small Cross\u2010Sectional Study in a Rural Area of Madagascar"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7221-0010","authenticated-orcid":false,"given":"Adrien","family":"Rieux","sequence":"first","affiliation":[{"name":"CIRAD, UMR PVBMT, F\u201097410 St Pierre  La R\u00e9union France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamitina Alain Noah","family":"Rabenandrasana","sequence":"additional","affiliation":[{"name":"Experimental Bacteriology Unit Institut Pasteur de Madagascar  Antananarivo Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2017.06.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13060551"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000192"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01555-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020292"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_8_1","unstructured":"Cattoir V.2024. 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V.1.0.\u201dwww.sfm\u2010microbiologie.org."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad125"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ve\/vez039"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2018.2501"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.01125-20"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.arba\u20100009\u20102017"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.786146"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2142-7"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.0332"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02227-15"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0524-x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfp.2024.100368"},{"key":"e_1_2_9_24_1","article-title":"RFPlasmid: 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