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Heavy\u2010metal\u2010contaminated rhizospheres were sequentially exposed to ex\u2010situ and in\u00a0vitro enrichment with heavy metals from battery waste and incubated for 168\u2009h. The surviving microbiota were screened against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. baumannii<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and crude extracts of high\u2010performing strains were tested against the pathogens using agar well diffusion assays. The novelty and components of the extracted secondary metabolites from environmentally stressed rhizospheric microorganisms were inferred using ultra\u2010performance liquid chromatography\u2010high resolution mass spectrometry (UPLC\u2010HRMS). Results indicated that these secretions inhibited the growth of XDR pathogens (approximately 3.0\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    \u2009CFU\/mL), forming inhibition zones of up to 50\u2009mm. Furthermore, the pathogens were more responsive to exudates from microbiota in environmentally stressed rhizospheres compared to those from organic rhizospheres (control). Heavy\u2010metal\u2010stressed microbiota secrete metabolites that show superior antimicrobial activity and successfully inhibit the growth of XDR pathogens. The UPLC\u2010HRMS analysis indicated the tentative characterisation of the metabolites, particularly\n                    <jats:italic>Tolyposamine<\/jats:italic>\n                    and\n                    <jats:italic>Gentiatibetine<\/jats:italic>\n                    , by the evaluated microbiota, suggesting their relevance as biopharmaceuticals, and could lead to future antibiotic production.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70182","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T13:36:07Z","timestamp":1758202567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bioprospecting Heavy\u2010Metal Rhizospheres for Novel Therapeutics Against High\u2010Priority Multi\u2010Drug\u2010Resistant\n                    <scp>\n                      <i>Pseudomonas aeruginosa<\/i>\n                    <\/scp>\n                    and\n                    <scp>\n                      <i>Acinetobacter baumannii<\/i>\n                    <\/scp>\n                    : A Case of Toxic to Treatment"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1243-4996","authenticated-orcid":false,"given":"Kylah B.","family":"Millard","sequence":"first","affiliation":[{"name":"Sustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT) University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8096-1156","authenticated-orcid":false,"given":"John O.","family":"Unuofin","sequence":"additional","affiliation":[{"name":"Sustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT) University of Pretoria  Pretoria South 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