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In contrast to alfalfa bacteroids, bean (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Phaseolus vulgaris<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) bacteroids exhibit high levels of alkaline phosphatase (AP), the native reporter enzyme for the bacterial Pi stress response.\n                    <jats:sup>14<\/jats:sup>\n                    C and\n                    <jats:sup>32<\/jats:sup>\n                    Pi whole plant labelling techniques were used in conjunction with diagnostic mutants (lacking AP or lacking high affinity Pi transport) to assess the relative importance of the Pi stress response in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Rhizobium tropici<\/jats:italic>\n                    <\/jats:styled-content>\n                    bacteroids during symbiosis. The AP\u2010 mutant was not defective for symbiosis and did not differ from wildtype bacteroids for Pi acquisition.\n                    <jats:sup>14<\/jats:sup>\n                    C\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    feeding to host plants revealed\n                    <jats:sup>14<\/jats:sup>\n                    C\u2010carbon uptake and accumulation in AP\u2010 mutant bacteroids, and their nodules were increased relative to wildtype bacteroids, implying that organo\u2010P compounds may account for meaningful levels of carbon exchange between symbionts.\n                    <jats:sup>32<\/jats:sup>\n                    Pi tracer experiments implied that the high affinity transporter is important to bacteroid Pi acquisition and symbiotic performance in determinate nodules, but that the symbiosome Pi concentration does not meet the capacity of the high affinity transporter.\n                    <jats:sup>32<\/jats:sup>\n                    P tracer work also illustrated that Pi taken up into the nodule does not remain in the nodule, but rather is redistributed to the host.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70220","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T02:14:56Z","timestamp":1762136096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Observations Concerning\n                    <scp>\n                      <i>Rhizobium tropici<\/i>\n                    <\/scp>\n                    Bacteroid Phosphorus Stress Response During Symbiosis With\n                    <scp>\n                      <i>Phaseolus vulgaris<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"17","author":[{"given":"Lina M.","family":"Botero","sequence":"first","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamir","family":"Al\u2010Niemi","sequence":"additional","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8293-5106","authenticated-orcid":false,"given":"Timothy R.","family":"McDermott","sequence":"additional","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.4.1233"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004200903458"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.1.15-22.2000"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj1981.03615995004500030016x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2134\/agronj1981.00021962007300010005x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100311"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050610"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195894"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.89.1.225"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511600395.003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.017004"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000254"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.000745"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.143842"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-02-18-0031-R"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.84.3.835"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1993.tb05502.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/00380768.1987.10557587"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1985.tb02334.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.1.19"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.1.31"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12468"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1081-1088.2006"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-004-1030-8"},{"key":"e_1_2_9_26_1","first-page":"45","volume-title":"Highlights of Nitrogen Fixing Research","author":"McDermott T. 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