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Here, we describe a novel carboxydotrophic halo\u2010alkaliphilic, acetogenic bacterium, strain MD4, isolated from a CO\u2010fed bioreactor operated under high salt and alkaline conditions. Phylogenetic analysis suggests that strain MD4 is the first representative of a novel genus, branching between the Alkalibacter and Alkalibaculum genera, for which we propose the name\n                    <jats:italic>Haloacetibacter carboxydivorans<\/jats:italic>\n                    . The bacterium tolerates a wide range of sodium (0.01\u20132.5\u2009M) and pH (7\u201310), but was not exceptionally tolerant to metals such as copper, nickel and cobalt. During growth on CO, strain MD4 produced formate and acetate, the former being co\u2010consumed upon low CO availability to drive acetogenesis. Interestingly, common by\u2010products of carboxydotrophic acetogenesis\u2014ethanol or hydrogen\u2014were not produced, suggesting that formate production may serve as a form of redox homeostasis during alkaliphilic carboxydotrophy. Genome analyses revealed no clear bifurcating formate dehydrogenase or formate hydrogen lyase, but during carboxydotrophy the transcriptome showed high expression of two putative bifurcating hydrogenases, and a NADH\u2010dependent formate dehydrogenase, potentially playing a role in the dynamic formate metabolism.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70254","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:14:11Z","timestamp":1769505251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carboxydotrophic Acetogenesis in Alkaline Conditions Results in Transient Formate Production by the Halo\u2010Alkaliphilic Acetogen\n                    <i>Haloacetibacter carboxydivorans<\/i>\n                    Gen. Nov. sp. Nov"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0408-688X","authenticated-orcid":false,"given":"Martijn","family":"Diender","sequence":"first","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"},{"name":"Centre for Living Technologies, EWUU Alliance  Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6952-263X","authenticated-orcid":false,"given":"Isabelle M.","family":"Keijsers","sequence":"additional","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0533-6600","authenticated-orcid":false,"given":"Anastasia","family":"Galani","sequence":"additional","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3342-6513","authenticated-orcid":false,"given":"Timo","family":"van Roosmalen","sequence":"additional","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7840-6500","authenticated-orcid":false,"given":"Alfons J. M.","family":"Stams","sequence":"additional","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3569-1545","authenticated-orcid":false,"given":"Diana Z.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Wageningen University &amp; Research  Wageningen the Netherlands"},{"name":"Centre for Living Technologies, EWUU Alliance  Utrecht the 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