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Nitric oxide and nitrite also act as aerobic respiratory inhibitors.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    must overcome these toxic metabolites alongside self\u2010produced cyanide to persist at the infection site. We previously identified a novel nitrite reductase (NirA) that supports\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    virulence in a wide range of infection models. In this work, we demonstrate that mutation of\n                    <jats:italic>nirA<\/jats:italic>\n                    inhibits growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    at reduced oxygen tensions in the presence of nitrite or nitrate, with this phenotype shown to be dependent on cyanide. NirA is a siroheme\u2010dependent enzyme, a classical target for inhibition with cyanide. Biochemical characterisation confirms that NirA is a novel cyanide\u2010tolerant nitrite reductase, which supports reduction of nitrite in the presence of cyanide. We hypothesise that NirA enables detoxification of nitrite to prevent build\u2010up of multiple respiratory inhibitors and facilitate cyanide\u2010resistant aerobic respiration at low oxygen tensions. Through targeting effectors of these resistance mechanisms, we could promote\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    self\u2010poisoning and prevent adaptation to the reduced oxygen environment typically encountered by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    in biofilms and during infection.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70256","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T03:00:56Z","timestamp":1765335656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>NirA<\/scp>\n                    is a Cyanide\u2010Tolerant Nitrite Reductase Which Protects\n                    <scp>\n                      <i>Pseudomonas aeruginosa<\/i>\n                    <\/scp>\n                    From Self\u2010Poisoning"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0522-8894","authenticated-orcid":false,"given":"Samuel","family":"Fenn","sequence":"first","affiliation":[{"name":"National Biofilms Innovation Centre, Biodiscovery Institute, School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Biosciences Institute, School of Microbiology University College Cork  Cork Ireland"},{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1844-457X","authenticated-orcid":false,"given":"Carey","family":"Lambert","sequence":"additional","affiliation":[{"name":"National Biofilms Innovation Centre, Biodiscovery Institute, School of Life Sciences University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2422-6495","authenticated-orcid":false,"given":"Dimitra","family":"Panagiotopulou","sequence":"additional","affiliation":[{"name":"National Biofilms Innovation Centre, Biodiscovery Institute, School of Life Sciences University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-4039","authenticated-orcid":false,"given":"Ruth","family":"Massey","sequence":"additional","affiliation":[{"name":"Biosciences Institute, School of Microbiology University College Cork  Cork Ireland"},{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4585-7385","authenticated-orcid":false,"given":"Miguel","family":"C\u00e1mara","sequence":"additional","affiliation":[{"name":"National Biofilms Innovation Centre, 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