{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T03:21:32Z","timestamp":1787714492131,"version":"build-2784847793"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Vibrio splendidus<\/jats:italic>\n                    <\/jats:styled-content>\n                    is an opportunistic pathogen widely distributed in marine environments, animal tissues and seabed sediments. It can infect various marine animals, resulting in high mortality and substantial economic losses. Exposure to high concentrations of tetracycline induces\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. splendidus<\/jats:italic>\n                    <\/jats:styled-content>\n                    to form persister cells, which exhibit tolerance to multiple classes of antibiotics and pose a serious threat to aquaculture by promoting disease outbreaks. Previous studies have suggested that metabolic regulation is a key mechanism for maintaining the dormant and low\u2010energy state of persister cells. In this study, we found that the accumulation of histidine\u2010derived metabolites significantly reduced the susceptibility of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. splendidus<\/jats:italic>\n                    <\/jats:styled-content>\n                    to tetracycline. Transcriptomic analysis revealed a marked upregulation of genes involved in histidine metabolism and exogenous supplementation of histidine significantly increased the proportion of persister cells, suggesting that histidine plays a promotive role in persister formation. Furthermore, histidine enhanced the membrane potential and upregulated the expression of the efflux pump gene\n                    <jats:italic>tolC<\/jats:italic>\n                    , thereby contributing to the formation of tetracycline\u2010induced persister cells. These findings reveal a previously unrecognized role of histidine metabolism in antibiotic tolerance and provide a theoretical basis for understanding persister cell formation in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. splendidus<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70383","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T23:34:33Z","timestamp":1782862473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Accumulation of Histidine Reduce the Susceptibility of\n                    <scp>\n                      <i>Vibrio splendidus<\/i>\n                    <\/scp>\n                    Persister Cells to Tetracycline"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4879-384X","authenticated-orcid":false,"given":"Yanan","family":"Li","sequence":"first","affiliation":[{"name":"School of Marine and Biological Engineering, Yancheng Teachers University  Yancheng Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinnan","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Marine and Biological Engineering, Yancheng Teachers University  Yancheng Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Marine and Biological Engineering, Yancheng Teachers University  Yancheng Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Ding","sequence":"additional","affiliation":[{"name":"GuXin Bioengineering Equipment (Jiangsu) Co. 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