{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T03:21:48Z","timestamp":1787714508948,"version":"build-2784847793"},"reference-count":93,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015747","name":"University Grants Commission of Bangladesh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015747","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    is a major nosocomial pathogen capable of developing multidrug resistance, forming biofilms, and disseminating antimicrobial resistance genes via mobile genetic elements. This study investigated the occurrence, antimicrobial resistance,\n                    <jats:italic>lecB<\/jats:italic>\n                    sequence diversity, and biofilm\u2010forming capacity of environmental\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates from three hospitals in Jashore, Bangladesh. Among 231 environmental samples collected from 29 sites in high\u2010risk hospital units, 36 isolates were confirmed by\n                    <jats:italic>lecB<\/jats:italic>\n                    gene sequencing. Sequence analysis classified 77.8% of isolates as PAO1\u2010like and 22.2% as PA14\u2010like, indicating genetic diversity. Biofilm formation was detected in 97.2% of isolates, with no significant difference between strain families. Phenotypic susceptibility testing revealed widespread multidrug resistance, with the highest resistance to ceftazidime (97.2%) and tetracycline (91.7%). Two operation theatre isolates (A40 and 2SW) exhibited extensive drug resistance within the tested antimicrobial panel. Polymerase chain reaction detected\n                    <jats:italic>blaSHV<\/jats:italic>\n                    (55.6%),\n                    <jats:italic>floR<\/jats:italic>\n                    (33.3%),\n                    <jats:italic>aac<\/jats:italic>\n                    (\n                    <jats:italic>6\u2032<\/jats:italic>\n                    )\n                    <jats:italic>\u2010Ib<\/jats:italic>\n                    (33.3%),\n                    <jats:italic>blaTEM<\/jats:italic>\n                    (27.8%), and\n                    <jats:italic>blaOXA\u201048<\/jats:italic>\n                    (11.1%), while class 1 integrons occurred in 55.6% of isolates and were associated with\n                    <jats:italic>floR<\/jats:italic>\n                    and\n                    <jats:italic>blaTEM<\/jats:italic>\n                    . Whole\u2010genome sequencing identified intrinsic resistance determinants and the mobile resistance genes\n                    <jats:italic>tmexCD\u2010toprJ<\/jats:italic>\n                    and\n                    <jats:italic>crpP<\/jats:italic>\n                    . These findings highlight genetically diverse, multidrug\u2010resistant, integron\u2010positive, biofilm\u2010forming\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    in Bangladeshi hospital environments, underscoring the need for routine environmental surveillance and strengthened infection prevention.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70391","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T01:39:46Z","timestamp":1784943586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence and Distribution of Environmental\n                    <i>Pseudomonas aeruginosa<\/i>\n                    From Hospitals in Bangladesh Reveals Diverse Strain Families, Multidrug Resistance, and Biofilm Formation"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5067-4043","authenticated-orcid":false,"given":"S. M.","family":"Kador","sequence":"first","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6434-5261","authenticated-orcid":false,"given":"Tanay","family":"Chakrovarty","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khandaker","family":"Adil","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4359-3411","authenticated-orcid":false,"given":"Md. Mustak","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Mohsin","family":"Kobir","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1263-2728","authenticated-orcid":false,"given":"Md.","family":"Hasanuzzaman","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mousufa","family":"Akter","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanzima Sharmim","family":"Taowhid","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8604-1035","authenticated-orcid":false,"given":"Md. Tanvir","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1114-3125","authenticated-orcid":false,"given":"Ovinu Kibria","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","unstructured":"Abdelraheem W. M. D. E.Ismail andS. S. J. B. I. 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