{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T03:22:24Z","timestamp":1787714544245,"version":"build-2784847793"},"reference-count":84,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201022\u2010CE02\u20100001"],"award-info":[{"award-number":["ANR\u201022\u2010CE02\u20100001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In deep\u2010sea hydrothermal vents, sulfur compounds are central for microbial bioenergetics. Despite its significant impact on the global sulfur cycle, anaerobic sulfur compound disproportionation remains the least understood metabolism, with only three bacterial strains having been investigated at the proteomic level for their sulfur disproportionation metabolism to date. Here, we present a comparative proteomics study of the chemolithoautotrophic sulfur\u2010cycling species\n                    <jats:italic>Dissulfuribacter thermophilus<\/jats:italic>\n                    S69\u1d40. Upon thiosulfate disproportionation conditions, proteomics revealed a high abundance of a cytoplasmic thiosulfate reductase AB homologue (PhsAB\u2010like) that might reductively cleave thiosulfate into sulfide and sulfite. Subsequent steps might involve enzymes from the dissimilatory sulfate reduction, with Apr and Sat potentially functioning in reverse to produce sulfate. Strain S69\n                    <jats:sup>T<\/jats:sup>\n                    exhibited enhanced growth under sulfite\/dihydrogen, likely due to both sulfite respiration and disproportionation. Here, a range of new enzymes were more abundant. These included an enzyme containing NrfD, the MolyAB as well as two membranous tetrathionate reductase homologues. Other membranous oxidoreductases such as QmoABC, DsrMKJOP and Complex I, may contribute to the pathway, while autotrophic growth is likely sustained by the Wood\u2013Ljungdahl pathway. These findings highlight the distinct enzymatic features of strain S69\u1d40 compared to previously proteomically characterised sulfur\u2010disproportionating strains, revealing novel enzymes that warrant further analysis.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70406","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T03:27:06Z","timestamp":1787196426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advancing the Resolution of the Sulfur Compound Disproportionation Puzzle in\n                    <i>Dissulfuribacter Thermophilus<\/i>\n                    S69\u1d40 Through Quantitative Label\u2010Free Comparative Proteomics"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7471-6189","authenticated-orcid":false,"given":"Marilina","family":"Fernandez","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech, Micalis Institute  Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6820-4478","authenticated-orcid":false,"given":"Barbara","family":"Schoepp\u2010Cothenet","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille Universit\u00e9, CNRS, BIP\u2010UMR 7281, IMM, IM2B  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4765-6483","authenticated-orcid":false,"given":"Marie","family":"Hemon","sequence":"additional","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, EMR 6002 BIOMEX, IRP 1211 MicrobSea, Unit\u00e9 Biologie et Ecologie Des Ecosyst\u00e8mes Marins Profonds BEEP  Plouzan\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3675-6267","authenticated-orcid":false,"given":"Carine","family":"Rodrigues\u2010Machado","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, INRAE, AgroParisTech, Micalis Institute  Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7773-9119","authenticated-orcid":false,"given":"Lukas V. F.","family":"Nov\u00e1k","sequence":"additional","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, EMR 6002 BIOMEX, IRP 1211 MicrobSea, Unit\u00e9 Biologie et Ecologie Des Ecosyst\u00e8mes Marins Profonds BEEP  Plouzan\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3555-633X","authenticated-orcid":false,"given":"Xavier","family":"Philippon","sequence":"additional","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, EMR 6002 BIOMEX, IRP 1211 MicrobSea, Unit\u00e9 Biologie et Ecologie Des Ecosyst\u00e8mes Marins Profonds BEEP  Plouzan\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0707-717X","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Laurent","sequence":"additional","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, EMR 6002 BIOMEX, IRP 1211 MicrobSea, Unit\u00e9 Biologie et Ecologie Des Ecosyst\u00e8mes Marins Profonds BEEP  Plouzan\u00e9 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