{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:51:00Z","timestamp":1791330660596,"version":"4.3.1"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Microbial communities drive the stabilisation of organic matter during manure storage; however, the combined influence of substrate origin and sudden rainfall occurrences on these communities remains poorly understood. For the first time in Cyprus, we investigated the effects of manure type (dairy cow vs. goat) and a drying\u2013rainfall simulation pulse on the community composition, diversity, and functional potential during manure storage, using 16S rRNA metabarcoding and shotgun sequencing. Under dry conditions, substrate composition set the baseline, with the more structurally heterogeneous goat manure supporting significantly higher diversity than dairy cow manure. Rainfall simulation then acted as a dominant environmental filter, sharply reducing diversity and driving compositional convergence across manure types. This shift coincided with a rapid rise of fast\u2010responding, stress\u2010tolerant taxa, primarily Bacillota, and an increase in functions linked to sporulation and rapid growth. Ordination and dispersion analyses further showed that substrate origin modulates stability: after rainfall simulation, cow\u2010manure communities exhibited greater compositional displacement and greater variability than those from goat manure. Overall, the efficacy and stability of manure storage systems emerge from the interplay between initial physicochemical template and dynamic moisture stress. Optimising large\u2010scale operations will require management approaches that consider substrate characteristics, microbial community structure, and moisture dynamics.<\/jats:p>","DOI":"10.1111\/1758-2229.70429","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:07:22Z","timestamp":1791328042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rainfall and Substrate Origin Driven Change of Ruminant Manure Microbiomes in Cyprus"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4841-7676","authenticated-orcid":false,"given":"P.","family":"Pissaridou","sequence":"first","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2823-6712","authenticated-orcid":false,"given":"K.","family":"Philippou","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1353-5106","authenticated-orcid":false,"given":"A.","family":"Phokas","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3178-1964","authenticated-orcid":false,"given":"J.","family":"Rodosthenous","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1829-7192","authenticated-orcid":false,"given":"C.","family":"Hadjimichael","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5526-0606","authenticated-orcid":false,"given":"M.","family":"Omirou","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnology Agricultural Research Institute  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","DOI":"10.3390\/agronomy13020542","article-title":"Microbial Community in the Composting Process and Its Positive Impact on the Soil Biota in Sustainable Agriculture","volume":"13","author":"Aguilar\u2010Paredes A.","year":"2023","journal-title":"Agronomy"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.geoderma.2008.08.007","article-title":"Past, Present and Future of Soil Quality Indices: A Biological Perspective","volume":"147","author":"Bastida F.","year":"2008","journal-title":"Geoderma"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"5444","DOI":"10.1016\/j.biortech.2008.11.027","article-title":"Composting of Animal Manures and Chemical Criteria for Compost Maturity Assessment. 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