{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T10:34:56Z","timestamp":1793097296102,"version":"4.5.0"},"reference-count":13,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007382","name":"Universidade Federal do Par\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007382","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312316\/2022\u20104"],"award-info":[{"award-number":["312316\/2022\u20104"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Environmental monitoring of contaminated freshwater systems still lacks curated genomic resources tailored to bioremediation\u2010oriented screening. In this study, we developed a curated catalogue of 546 genes from 80 microbial species associated with heavy\u2010metal resistance, hydrocarbon degradation, detoxification and related functions and applied it to river metagenomes from four municipalities in Par\u00e1, Brazilian Amazon: Altamira, Bragan\u00e7a, Marab\u00e1 and Paragominas. Functional annotation was performed using eggNOG\u2010mapper and metagenomic reads were aligned against the curated catalogue with Bowtie2. Mercury\u2010 and copper\u2010related genes were the most frequently represented functional categories across all river systems, a pattern that may reflect both sequence representation in the metagenomes and the unequal composition of the curated reference catalogue. Bragan\u00e7a showed the largest detected gene repertoire, whereas diversity, Jaccard, UpSet and Dunn\u2010based analyses revealed marked spatial heterogeneity and sites of localized functional enrichment. Recurrently associated bacterial taxa included\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Cupriavidus<\/jats:italic>\n                    and\n                    <jats:italic>Sphingopyxis<\/jats:italic>\n                    . These findings indicate that the catalogue provides a targeted genomic reference for environmental screening and bioprospecting, although metagenomic detection represents functional potential rather than experimentally confirmed remediation activity.\n                  <\/jats:p>","DOI":"10.1111\/1758-2229.70435","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:59:58Z","timestamp":1792623598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic Bioprospecting of Bioremediation\u2010Related Genes in Amazonian Aquatic Ecosystems"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8047-0437","authenticated-orcid":false,"given":"Thais Mayara Castro","family":"dos Anjos","sequence":"first","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and Computational Biology, High Performance Computing Center Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0186-8149","authenticated-orcid":false,"given":"Daralyns Borges","family":"Macedo","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and Computational Biology, High Performance Computing Center Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3637-5544","authenticated-orcid":false,"given":"Victor Benedito Costa","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and Computational Biology, High Performance Computing Center Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3681-7886","authenticated-orcid":false,"given":"Carlos Willian Dias","family":"Dantas","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and Computational Biology, High Performance Computing Center Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9715-9437","authenticated-orcid":false,"given":"Edian Franklin Franco","family":"de Los Santos","sequence":"additional","affiliation":[{"name":"Santo Domingo Institute of Technology  Santo Domingo Dominican Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar Victor","family":"Cardenas\u2010Alegria","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and Computational Biology, High Performance Computing Center Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8032-1474","authenticated-orcid":false,"given":"Rommel Thiago Juc\u00e1","family":"Ramos","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics and Genomics of Microorganisms Institute of Biological Sciences, Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Simulation and 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