{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:36:25Z","timestamp":1792121785558,"version":"4.4.0"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T00:00:00Z","timestamp":1374624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The aim of this study was to evaluate the cytotoxic and antitumor activity of spider venom (SV<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Cell proliferation and cytotoxicity were determined by 3<\/jats:sup>H<\/jats:styled-content>\u2010methyl thymidine incorporation ([3<\/jats:sup>H<\/jats:styled-content>]\u2010TDR<\/jats:styled-content>) assay. DNA<\/jats:styled-content> fragmentation and cell cycle kinetics were analyzed by FACS<\/jats:styled-content>. In vivo<\/jats:italic> inhibition of tumor size of nude mice by SV<\/jats:styled-content> (5.0, 10.0, 20.0\u2009mg\/kg mice) was constructed.<\/jats:p><\/jats:sec>Results<\/jats:title>SV<\/jats:styled-content> exhibited significant anti\u2010cancer effects on human squamous esophageal carcinoma cells TE13<\/jats:styled-content>, mainly as a result of cell apoptosis induced by SV<\/jats:styled-content>. The anti\u2010cancer effects were likely achieved through decreasing [3<\/jats:sup>H<\/jats:styled-content>]\u2010TdR<\/jats:styled-content>. TE13<\/jats:styled-content> cells treated with SV<\/jats:styled-content> (25, 50, 100\u2009\u03bcg\/mL<\/jats:styled-content>), which were arrested in the G0<\/jats:sub>\/G1<\/jats:sub><\/jats:styled-content> phase. SV<\/jats:styled-content> treatment leads to anti\u2010proliferation effects, and significant apoptosis in TE13<\/jats:styled-content> cells with reactive oxygen species (ROS)<\/jats:styled-content> levels can increase dramatically and decrease cellular mitochondrial membrane potential (MMP<\/jats:styled-content>). In addition, Western blotting analysis showed that one of the pharmacological mechanisms of SV<\/jats:styled-content> was to activate the expression of P21<\/jats:styled-content>. In vivo<\/jats:italic> testing revealed that tumor size was significantly decreased after 21 days of treatment with the venom (P<\/jats:italic> < 0.01).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Our data showed that SVs<\/jats:styled-content> could inhibit TE13<\/jats:styled-content> cell proliferation in vitro<\/jats:italic> and in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.12020","type":"journal-article","created":{"date-parts":[[2012,12,14]],"date-time":"2012-12-14T12:23:07Z","timestamp":1355487787000},"page":"306-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of spider venom on inhibition proliferation of TE13<\/scp> cells in vivo<\/i> and in vitro<\/i>"],"prefix":"10.1111","volume":"4","author":[{"given":"Li","family":"Gao","sequence":"first","affiliation":[{"name":"College of Life Science Hebei Normal University Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation The Fourth Hospital of Hebei Medical University Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyan","family":"Liu","sequence":"additional","affiliation":[{"name":"The Department of Oncology Hebei Province Chest Hospital Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Department of Oncology Hebei Province Chest Hospital Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Information and Technical Hebei Normal University Shijiazhuang 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effect of the venom of spider Macrothele raveni on proliferation of human hepatocellular carcinoma cell line BEL\u20107402 and its mechanism","volume":"24","author":"Gao L","year":"2005","journal-title":"Ai Zheng"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1759-7714.2010.00009.x"}],"container-title":["Thoracic 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